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Alexa Fluor™ 647 IgG1, κ N297A Human Isotype Control

BD Pharmingen™ Alexa Fluor™ 647 IgG1, κ N297A Human Isotype Control

Clone X40.huIgG1N297A.rMAb (also known as X40.huIgG1 N297A.rMAb)

(RUO)
Product Details
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BD Pharmingen™
IgG1, kappa Isotype Control (Anti-KLH)
Human IgG1, κ
Keyhole limpet hemocyanin (KLH)
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  10. For U.S. patents that may apply, see bd.com/patents.
569794 Rev. 2
Antibody Details
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X40.huIgG1N297A.rMAb

X40.huIgG1N297A.rMAb is a chimeric recombinant monoclonal antibody that specifically recognizes keyhole limpet hemocyanin (KLH), an antigen not expressed by mammalian cells. This antibody was engineered using variable domain sequences from the original KLH-specific X40 clone with constant region sequences derived from Human IgG1, κ. Asparagine at position 297 is replaced with alanine (N297A) to further reduce Fc receptor interactions of this reagent. The X40.huIgG1N297A.rMAb can thus serve as a Human IgG1, κ Isotype Control for antibodies with the Human IgG1, κ isotype and N297A mutation.

Note: For flow cytometric analysis, Alexa Fluor™ 647 IgG1, κ N297A Human Isotype Control (Cat. No. 569961) is intended for staining nonpermeabilized cells whereas Alexa Fluor™ 647 IgG1, κ N297A Human Isotype Control (Cat. No. 569794) is intended for staining fixed and permeabilized cells.

569794 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
569794 Rev.2
Citations & References
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View product citations for antibody "569794" on CiteAb
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569794 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.