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BD Phosflow™ Alexa Fluor® 488 Mouse IgG1 κ Isotype Control
Clone MOPC-21 (also known as MOPC21; MOPC 21) (RUO)
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
Flow cytometric analysis on U-937 cells. U-937 cells (ATCC CRL-1593) were serum starved overnight (RPMI containing 0.1% FCS). The following day cells were either left untreated (solid line) or treated (dotted line) with recombinant human IFN-γ (Cat. No. 554617; 1000 U/ml for 10 minutes at 37°C). Cells were then fixed in 2% paraformaldehyde (10 minutes at 37°C) and permeabilized by adding 90% methanol to the cell pellet (30 minutes on ice or overnight at -20°C). Cells were washed twice in BD Pharmingen™ Stain Buffer and stained with the Alexa Fluor® 488 Mouse IgG1, κ Isotype Control antibody (1 hour at RT; Cat. No. 557782). The cells were analyzed on a BD FACSCalibur™ flow cytometry system.
Product Details
BD Phosflow™
Human (QC Testing)
Mouse IgG1, κ
Intracellular staining (flow cytometry), Isotype control (Routinely Tested)
20 µl
AB_396870
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
- The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
- Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
557782 Rev. 4
Antibody Details
MOPC-21
The MOPC-21 immunoglobulin is a mouse myeloma protein. The MOPC-21 immunoglobulin was selected as an isotype control following screening for low background on a variety of mouse and human tissues.
557782 Rev. 4
Format Details
Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
494 nm
517 nm
557782 Rev.4
Citations & References
View product citations for antibody "557782" on CiteAb
No Citations Are Available for this Product
557782 Rev. 4
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.