PE Mouse Anti-Human CD55

BD™ PE Mouse Anti-Human CD55

Name: PE Mouse Anti-Human CD55
Brand: BD™
SKU: 341030

Clone IA10

(RUO (GMP))

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Product Details

Brand: BD™

Alternative Name: DAF; CR; CROM; TC; Complement decay-accelerating factor

Reactivity: Human

Isotype: Mouse IgG2a, κ

Immunogen: Purified Human Decay Accelerating Factor

Application: Flow cytometry

Concentration: 25 μg/mL

Vol. Per Test: 20 μL

Workshop Number: V BP352, S031

Entrez Gene ID: 1604

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

341030 Rev. 1

Antibody Details

IA10

The CD55 antibody, clone IA10, is derived from the fusion of X63Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with purified DAF.

The CD55 antibody reacts with decay-accelerating factor (DAF), a GPI-anchored single chain glycoprotein of approximately 70 kilodaltons (kDa). It has been suggested that the role of DAF is to protect cells from damage by autologous complement preventing the amplification steps of the complement cascade by interfering with the assembly of the C3 and the C5 convertases. The complement components in the formation of C3 convertases include: C4b2a (classical pathway) and C3bBb (alternative pathway). In the formation of C5 convertases, the complement components include: C4b2a3b (classical pathway) and C3bBb3b (alternative pathway).

341030 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

341030 Rev.1

Citations & References

View product citations for antibody "341030" on CiteAb

Development References (8)

Bessler M, Mason PJ, Hillmen P, et al. Paroxysmal nocturnal haemoglobinuria (PNH) is caused by somatic mutations in the PIG-A gene. EMBO J. 1994; 13:110-117. (Biology).
Brodbeck WG, Kuttner-Kondo L, Mold C, Medof ME. Structure/function studies of human decay-accelerating factor. Immunology. 2000; 101:104-111. (Biology).
Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
Clinical and Laboratory Standards Institute. 2005. (Biology).
Hall SE, Rosse WF. The use of monoclonal antibodies and ﬂow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria. Blood. 1996; 87:5332-5340. (Biology).
Klickstein LB, Springer TA. Schlossman SF, Boumsell L, Gilks W, et al, ed. Leucocyte Typing V: White Cell Differentiation Antigens. New York, NY: 1995:1473-1474.
Kuby J. Immunology, ed. New York, NY: WH Freeman and Co; 1994:398-401.
Richards SJ, Rawstron AC, Hillmen P. Application of ﬂow cytometry to the diagnosis of paroxysmal nocturnal hemoglobinuria. Cytometry. 2000; 42:223-233. (Biology).

View All (8)

341030 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Although not required, these products are manufactured in accordance with Good Manufacturing Practices.