FITC Mouse Anti-Human ZAP-70

BD™ FITC Mouse Anti-Human ZAP-70

Name: FITC Mouse Anti-Human ZAP-70
Brand: BD™
SKU: 344931

Clone 1E7.2

(RUO (GMP))

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Product Details

Brand: BD™

Reactivity: Human

Isotype: Mouse IgG1, κ

Immunogen: Human

Application: Flow cytometry

Concentration: 12.5 μg/mL

Vol. Per Test: 20 μL

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: RUO (GMP)

Preparation And Storage

The FITC and PE conjugates are each supplied as 12.5 μg in 1.0 mL (12.5 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

344931 Rev. 1

Antibody Details

1E7.2

Anti–ZAP-70, clone 1E7.2, is generated from the fusion of AG8.553 myeloma cells with spleen cells from BALB/c mice immunized with a ZAP-70 peptide 282-307 conjugated to KLH.

Anti–ZAP-70 is a 70-kilodalton (kd) protein kinase, a member of the Syk family of protein tyrosine kinases that is associated with the zeta chain of the CD3/TCR complex. Anti–ZAP-70 plays a crucial role in T-cell signal transduction and thymocyte development.

344931 Rev. 1

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

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Citations & References

Development References (7)

Chan AC, Iwashima M, Turch CW, Weiss A. ZAP-70: a 70 kd protein-tyrosine kinase that associates with the TCR ζ chain. Cell. 1992; 71:649-662. (Biology).
Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol. 1999; 163(5):2610-2620. (Biology). View Reference
Crespo M, Bosch F, Villamor N, et al. ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med. 2003; 348:1764-1775. (Biology).
Orchard JA, Ibbotson RE, Davis Z, et al. ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. Lancet. 2004; 363:105-111. (Biology).
Qian D, Lev S, van Oers NSC, Dikic I, Schlessinger J, Weiss A. Tyrosine phosphorylation of Pyk2 is selectively regulated by Fyn during TCR signaling. J Exp Med. 1997; 185:1253-1259. (Biology).
Rassenti LZ, Huynh L, Toy TL, et al. ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. N Engl J Med. 2004; 351:893-901. (Biology).
Straus DB, Weiss A. The CD3 chains of the T cell antigen receptor associate with the ZAP-70 tyrosine kinase and are tyrosine phosphorylated after receptor stimulation. J Exp Med. 1993; 178:1523-1530. (Biology).

View All (7)

344931 Rev. 1

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