CD8 PE

BD™ CD8 PE

Name: CD8 PE
Brand: CD8 PE
SKU: 345773

Clone SK1 (also known as Leu-2a; Leu2a) (CE_IVD)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD™

Alternative Name: CD8α; CD8A; CD8 alpha; Leu2a; MAL; T8; p32

Reactivity: Human

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Human Peripheral Blood T Cells

Application: Flow cytometry

Concentration: 12.5 µg/mL

Vol. Per Test: 20 μL

Workshop Number: I T51,74; III T118,152,571

Entrez Gene ID: 925

Storage Buffer: Phosphate buffered saline with gelatin and 0.1% sodium azide.

Regulatory Status: CE_IVD

Preparation And Storage

The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the outside of the reagent vial dry.

Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.

345773 Rev. 1

Antibody Details

SK1

CD8 is intended for in vitro diagnostic use in the identiﬁcation of cells expressing CD8 antigen, using a BD FACS™ brand ﬂow cytometer. The ﬂow cytometer must be equipped to detect light scatter and the appropriate ﬂuorescence, and be equipped with appropriate analysis software for data acquisition and analysis. Refer to your instrument user’s guide for instructions.

345773 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

345773 Rev.1

Citations & References

View product citations for antibody "345773" on CiteAb

Development References (1)

Engleman EG, Benike CJ, Glickman E, Evans RL. Antibodies to membrane structures that distinguish suppressor/cytotoxic and helper T lymphocyte subpopulations block the mixed leukocyte reaction in man. J Exp Med. 1981; 154(1):193-198. (Biology). View Reference

345773 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.