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Purified Mouse Anti-pICIn
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Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat, Dog (Tested in Development)
Mouse IgG1
Human pICln aa. 92-201
Western blot (Routinely Tested)
37/42 kDa
250 µg/ml
AB_398802
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
611274 Rev. 1
Antibody Details
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32/pICln

pICln (I = current, Cl = chloride, n = nucleotide-sensitive) was initially described as an integral component of a nucleotide-sensitive chloride channel. However, it lacks homology to known channel proteins and exhibits cytoplasmic and nuclear localization. Thus, pICln is thought to be a cytosolic regulator of a swelling-induced chloride channel, rather than a component of a channel itself. In support of this hypothesis, pICln interacts with several cytosolic proteins. It binds to several Sm proteins, which are common spliceosomal core proteins whose assembly is necessary for the biogenesis of small nuclear ribonucleoproteins (snRNPs). Sm protein association with pICln inhibits their assembly. pICln also interacts with the 72 kDa human homolog of Skb1, a yeast protein that binds a homolog of p21[Cdc42/Rac]-activated protein kinases. In addition, pICln interacts with, and is phosphorylated by, a constitutively active cytosolic protein kinase. Therefore, pICln is thought to be a component of signaling pathways that function in transcriptional or translational regulation, cytoskeletal reorganization, or a number of other biological processes.

611274 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611274 Rev.1
Citations & References
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View product citations for antibody "611274" on CiteAb

Development References (3)

  1. Krapivinsky G, Pu W, Wickman K, Krapivinsky L, Clapham DE. pICln binds to a mammalian homolog of a yeast protein involved in regulation of cell morphology. J Biol Chem. 1998; 273(18):10811-10814. (Biology). View Reference
  2. Pu WT, Krapivinsky GB, Krapivinsky L, Clapham DE. pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol Cell Biol. 1999; 19(6):4113-4120. (Biology). View Reference
  3. Sanchez-Olea R, Emma F, Coghlan M, Strange K. Characterization of pICln phosphorylation state and a pICln-associated protein kinase. Biochim Biophys Acta. 1998; 1381(1):49-60. (Biology). View Reference
611274 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.