Preparation And Storage
Recommended Assay Procedures
Applications include western blot analysis (1-2 µg/ml) and immunoprecipitation (1-2 µg/1x10 [6] cells). Immunoprecipitation application is not routinely tested, only tested during development.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
The T-cell receptor (TCR), expressed by thymus-derived lymphocytes, is a multicomponent complex responsible for recognizing antigen in the context of MHC molecules. Ti is the antigen-specific binding component of the TCR. On the majority of peripheral T lymphocytes, Ti is present as a disulfide-linked α-β heterodimer. A minor fraction express Ti as a γ-δ heterodimer. The Ti subunits are Ig-like, each containing V and C regions. Ti is noncovalently associated with an invariant set of molecules referred to as the CD3 subunits. CD3 appears early in thymocyte differentiation and remains expressed on all mature T lymphocytes. In the mouse, it consists of five chains: γ, δ, ε, ζ, and η. The relative masses of the mouse CD3 chains are 21, 28, 26, 32, (dimer) and 21 kDa, respectively. Clone 1ζ3A1 recognizes an epitope in the C-terminus of mouse CD3ζ. A synthetic peptide corresponding to the C-terminus of mouse CD3ζ (BSACDTYDALHMQTLAPR) was used as immunogen. Hybridomas were derived from Balb/c spleen cells x NS-1.
Development References (1)
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Hussey RE, Clayton LK, Diener A, et al. Overexpression of CD3 eta during thymic development does not alter the negative selection process. J Immunol. 1993; 150(4):1183-1194. (Immunogen: Immunoprecipitation, Western blot). View Reference
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
