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Purified Mouse Anti- APP-BP1
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Product Details
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BD Transduction Laboratories™
Amyloid Precursor Protein-Binding Protein 1
Mouse (QC Testing), Human, Rat, Dog (Tested in Development)
Mouse IgG2a
Human APP-BP1 aa. 348-464
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
59 kDa
250 µg/ml
AB_399345
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611865 Rev. 1
Antibody Details
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20/APP-BP1

Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. These proteins are processed into β-Amyloid peptides (39-43 amino acids), which form abnormal plaques in the cerebral cortex and blood vessel walls during Alzheimer's disease. The transmembrane form of APP is a putative cell surface receptor that possesses neurite-promoting activity, co-localizes with the plaques found in Alzheimer's disease, and is involved in synaptic vesicle recycling. APP binding protein 1 (APP-BP1) interacts with the C-terminus of APP, and is a relative of the ubiquitin-activating enzymes (E1). The structure of APP-BP1 includes a human Uba3 binding site (UBS) at amino acids 443 to 479. This site may be important for APP-BP1 regulation of the cell cycle through interactions with ubiquitinylation-related pathways. Transfection of APP-BP1 in cells with a ts41 mutation suppresses the abnormal S-phases observed in these cells in a hUba3- and hUbc12-dependent manner. In addition, APP-BP1 has been implicated in ubiquitinylation-dependent apoptosis in neurons. Thus, APP-BP1 may be a multi-functional APP-binding protein that regulates cell cycle dynamics.

611865 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611865 Rev.1
Citations & References
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View product citations for antibody "611865" on CiteAb

Development References (3)

  1. Chen Y, McPhie DL, Hirschberg J, Neve RL. The amyloid precursor protein-binding protein APP-BP1 drives the cell cycle through the S-M checkpoint and causes apoptosis in neurons. J Biol Chem. 2000; 275(12):8929-8935. (Biology). View Reference
  2. Chow N, Korenberg JR, Chen XN, Neve RL. APP-BP1, a novel protein that binds to the carboxyl-terminal region of the amyloid precursor protein. J Biol Chem. 1996; 271(19):11339-11346. (Biology). View Reference
  3. Hori T, Osaka F, Chiba T. Covalent modification of all members of human cullin family proteins by NEDD8. Oncogene. 1999; 18(48):6829-6834. (Biology). View Reference
611865 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.