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Purified Mouse Anti-Human Stat2
Purified Mouse Anti-Human Stat2
Western blot analysis of Stat2 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human Stat2 antibody.
Western blot analysis of Stat2 on a K-562 cell lysate (Human bone marrow myelogenous leukemia; ATCC CCL-243).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-human Stat2 antibody.
Product Details
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BD Transduction Laboratories™
Human (QC Testing)
Mouse IgG2a
Human Stat2 aa. 1-178
Western blot (Routinely Tested), Immunoprecipitation (Tested During Development), Immunofluorescence, Immunohistochemistry (Not Recommended)
113 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610187 Rev. 1
Antibody Details
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The Stat proteins function as both cytoplasmic signal transducers and activators of transcription. Stat91/84 (the two proteins are the result of alternate splicing-Stat91 has an additional 38 C-terminal amino acids) and Stat113 were the first identified members of this protein family. These three polypeptides contain both SH2 and SH3 domains and have also been described as members of the ISGF3 (interferon-stimulated gene factor 3) complex. With the discovery of additional members of the Stat family (Stats3 & 4), the nomenclature has been revised to indicate the Stat family members in the order of their discovery. Stat 91, 84, and 113 have become Stat1α, Stat1β, and Stat2, respectively. In response to IFN-α treatment, Stat1α, Stat1β, and Stat2 become tyrosine-phosphorylated and migrate to the nucleus where they join a 48 kDa DNA binding protein and subsequently direct the transcription at IFN-α responsive elements. The genes encoding Stat2 and Stat1 are closely related. An extended acidic C-terminal region exclusive to Stat2 implicates a potential role in gene activation. Stat2 phosphorylation can occur independently of Stat1α, but phosphorylated Stat2  does appear to be dependent on its interaction with Stat1α for efficient nuclear localization and/or stability.

610187 Rev. 1
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
610187 Rev.1
Citations & References
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Development References (5)

  1. Blesofsky WA, Mowen K, Arduini RM. Regulation of STAT protein synthesis by c-Cbl. Oncogene. 2001; 20(50):7326-7333. (Biology: Western blot). View Reference
  2. Duff JL, Quinlan KL, Paxton LL, Naik SM, Caughman SW. Pervanadate mimics IFNgamma-mediated induction of ICAM-1 expression via activation of STAT proteins. J Invest Dermatol. 1997; 3(295):2301. (Biology: Gel shift). View Reference
  3. Fu XY, Schindler C, Improta T, Aebersold R, Darnell JE Jr. The proteins of ISGF-3, the interferon alpha-induced transcriptional activator, define a gene family involved in signal transduction. Proc Natl Acad Sci U S A. 1992; 89(16):7840-7843. (Biology). View Reference
  4. Ruff-Jamison S, Chen K, Cohen S. Induction by EGF and interferon-gamma of tyrosine phosphorylated DNA binding proteins in mouse liver nuclei. Science. 1993; 261(5129):1733-1736. (Biology). View Reference
  5. Russell-Harde D, Wagner TC, Rani MR. Role of the intracellular domain of the human type I interferon receptor 2 chain (IFNAR2c) in interferon signaling. Expression of IFNAR2c truncation mutants in U5A cells. J Biol Chem. 2000; 275(31):23981-23985. (Biology: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610187 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.