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Western blot analysis of TFIIB on a A431 cell lysate (Human epithelial carcinoma; ATCC CRL-1555). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the anti-TFIIB antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma; ATCC CCL-2.2).


BD Transduction Laboratories™ Purified Mouse Anti-TFIIB

BD Transduction Laboratories™ Purified Mouse Anti-TFIIB

Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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TFIIB is a ubiquitous factor required for transcription initiation by RNA polymerase II. Studies suggest that TFIIB serves as a bridge between the "TATA"-binding factor (TFIID) and RNA polymerase II during pre-initiation complex assembly and that TFIIB can be a target of acidic activators. An essential component of the machinery that transcribes protein-coding genes, the three-dimensional structure of the human TFIIB appears to consist of two direct repeats that adopt similar alpha-helical folds, conferring pseudo-twofold symmetry. An extensive, central basic surface, including an amphipathic alpha helix, is critical to the function of TFIIB as a bridge between the TBP-promoter complex and transcription factors. TFIIB has a calculated molecular weight of 33 kD and has been reported to be observed to migrate between 33-40 kD.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (4)
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Bagby S, Kim S, Maldonado E, Tong KI, Reinberg D, Ikura M. Solution structure of the C-terminal core domain of human TFIIB: similarity to cyclin A and interaction with TATA-binding protein. Cell. 1995; 82(5):857-867. (Biology). View Reference
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Lamberti C, Lin KM, Yamamoto Y, et al. Regulation of beta-catenin function by the IkappaB kinases. J Biol Chem. 2001; 276(45):42276-42286. (Biology: Immunofluorescence). View Reference
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Malik S, Hisatake K, Sumimoto H, Horikoshi M, Roeder RG. Sequence of general transcription factor TFIIB and relationships to other initiation factors. Proc Natl Acad Sci U S A. 1991; 88(21):9553-9557. (Biology). View Reference
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Pellizzoni L, Charroux B, Rappsilber J, Mann M, Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J Cell Biol. 2001; 152(1):75-85. (Biology: Western blot). View Reference
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