Purified Mouse Anti-RAFT1

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BD Transduction Laboratories™ Purified Mouse Anti-RAFT1

Name: Purified Mouse Anti-RAFT1
Brand: Purified Mouse Anti-RAFT1
SKU: 611133

Clone 30/RAFT1 (RUO)

Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma; ATCC CRL-1721). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RAFT1 antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

Western blot analysis of RAFT1 on a PC12 cell lysate (Rat neuroblastoma; ATCC CRL-1721). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti-RAFT1 antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma; ATCC CRL-1721).

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Product Details

Brand: BD Transduction Laboratories™

Alternative Name: Rapamycin And FKBP12 Target-1

Reactivity: Rat (QC Testing), Human, Mouse, Chicken (Tested in Development)

Isotype: Mouse IgG1

Immunogen: Rat RAFT1 aa. 185-290

Application: Western blot (Routinely Tested), Immunofluorescence (Tested During Development)

Target Molecular Weight: 245 kDa

Concentration: 250 µg/ml

RRID: AB_398443

Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Companion Products

PC12 Cell Lysate RUO

Size 500 µg Cat No. 611454

HRP Goat Anti-Mouse Ig RUO

Size 1 mL Cat No. 554002

FITC Goat Anti-Mouse Ig RUO

Size 0.5 mg Cat No. 554001

611133 Rev. 2

Antibody Details

30/RAFT1

Immunosuppressive agents such as cyclosporin A, FK506, and rapamycin are used routinely in clinical transplantation and the treatment of autoimmune disorders. Cyclosporin A and FK506 bind to cyclophilins and the FK-binding protein FKBP12, respectively. These complexes interact with, and inhibit the activity of, the calcineurin protein phosphatase, which is essential for T cell activation. Rapamycin also interacts with FKBP12, but this complex does not affect calcineurin. Instead, it inhibits signals required for G1 to S progression in yeast, in IL-2-stimulated T lymphocytes, and other mammalian cell types. Rapamycin-FKBP12 binds to a protein designated Rapamycin And FKBP12 Target-1 (RAFT1) in rats and FKBP-Rapamycin-Associated Protein (FRAP) in humans. RAFT1 is the homolog of the yeast genes TOR1 and TOR2 which, when mutated, lead to rapamycin resistance. RAFT1 is a PI4-kinase that exhibits homology with other PI kinases such as PI3-kinase and PI4-Kα. RAFT1 phosphorylates p70 S6 kinase and 4E-BP1 which are regulators of translational initiation. However, rapamycin-FKBP1 does not inhibit PI4-kinase activity and, therefore, affects RAFT1 activity via alternate mechanisms.

611133 Rev. 2

Format Details

Purified

Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.

Format: Purified

611133 Rev.2

Citations & References

View product citations for antibody "611133" on CiteAb

Development References (5)

Alarcon CM, Cardenas ME, Heitman J. Mammalian RAFT1 kinase domain provides rapamycin-sensitive TOR function in yeast. Genes Dev. 1996; 10(3):279-288. (Biology). View Reference
Burnett PE, Barrow RK, Cohen NA, Snyder SH, Sabatini DM. RAFT1 phosphorylation of the translational regulators p70 S6 kinase and 4E-BP1. Proc Natl Acad Sci U S A. 1998; 95(94):1432-1437. (Biology). View Reference
Castedo M, Ferri KF, Blanco J. Human immunodeficiency virus 1 envelope glycoprotein complex-induced apoptosis involves mammalian target of rapamycin/FKBP12-rapamycin-associated protein-mediated p53 phosphorylation. J Exp Med. 2001; 194(8):1097-1110. (Biology: Immunofluorescence, Western blot). View Reference
Sabatini DM, Erdjument-Bromage H, Lui M, Tempst P, Snyder SH. RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell. 1994; 78(1):35-43. (Biology). View Reference
Sabatini DM, Pierchala BA, Barrow RK, Schell MJ, Snyder SH. The rapamycin and FKBP12 target (RAFT) displays phosphatidylinositol 4-kinase activity. J Biol Chem. 1995; 270(36):20875-20878. (Biology). View Reference

View All (5)

611133 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.