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Purified Mouse Anti-Janusin
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Purified Mouse Anti-Janusin
Western blot analysis of Janusin on a rat cerebrum lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Janusin antibody.
Purified Mouse Anti-Janusin
Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).
Western blot analysis of Janusin on a rat cerebrum lysate.  Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-Janusin antibody.
Immunofluorescence staining of WI-38 cells (Human lung fibroblasts; ATCC CCL-75).
Product Details
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BD Transduction Laboratories™
Tenascin-R
Rat (QC Testing), Human, Mouse (Tested in Development)
Mouse IgG1
Human Janusin aa. 24-198
Western blot (Routinely Tested), Immunofluorescence (Tested During Development), Immunohistochemistry, Immunoprecipitation (Not Recommended)
180 & 160 kDa
250 µg/ml
AB_397961
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610629 Rev. 1
Antibody Details
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9/Janusin

Originally identified as the oligodendrocyte-specific glycoprotein J1-160/180, Janusin is a protein of 1356 amino acids found exclusively in the nervous system. It belongs to the family of extracellular matrix proteins that is similar to Tenascin. These proteins are important in maintaining the interactions between neurons and glial cells. The two different molecular sizes of Janusin result from alternative splicing of its mRNA. Although Tenascin is widely expressed, Janusin's mRNA expression peaks shortly after birth and is highest in the cerebellum, olfactory bulb, and hippocampus. Like Tenascin, Janusin has a fibrinogen-like domain, nine fibronectin type III domains, four epidermal growth factor-like repeats, and an amino terminal cystein-rich region.

610629 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610629 Rev.1
Citations & References
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View product citations for antibody "610629" on CiteAb

Development References (1)

  1. Fuss B, Wintergerst ES, Bartsch U, Schachner M. Molecular characterization and in situ mRNA localization of the neural recognition molecule J1-160/180: a modular structure similar to tenascin. J Cell Biol. 1993; 120(5):1237-1249. (Biology). View Reference
610629 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.