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Purified Mouse Anti-IκBε
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Product Details
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BD Transduction Laboratories™
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG2a
Human IκBε aa. 200-211
Western blot (Routinely Tested), Immunofluorescence (Tested During Development)
45 kDa
250 µg/ml
AB_398930
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
611408 Rev. 1
Antibody Details
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21/IkBe

NF-κB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It consists of homo- and heterodimeric proteins that belong to the Rel family of transcription factors. Rel proteins such as p50, p52, p65 (RelA), c-Rel, and RelB contain a Rel homology region (RHR) that includes DNA-binding and dimerization domains and a nuclear localization signal. Inactive NF-κB complexes are associated with their inhibitory IκB proteins. Several IκB proteins have been characterized including IκBα, IκBβ, and IκBε. They contain ankyrin repeats that interact with Rel RHR regions to form complexes in the cytoplasm that prevent nuclear translocation of NF-κB. In response to stimuli such as cytokines, LPS, and viral infections, IκB proteins are phosphorylated at critical residues by IκB kinases (IKKα and IKKβ) leading to proteolysis of IκB proteins. This frees the heterodimeric NF-κB to form a heterotetramer that translocates to the nucleus and acts as a transcriptional activator. IκBε may function primarily in the cytoplasm where it sequesters p65 and c-Rel, while IκBα and IκBβ have additional functions that involve entering the nucleus to inhibit Rel DNA binding.

611408 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
611408 Rev.1
Citations & References
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View product citations for antibody "611408" on CiteAb

Development References (4)

  1. Fischer C, Page S, Weber M, Eisele T, Neumeier D, Brand K. Differential effects of lipopolysaccharide and tumor necrosis factor on monocytic IkappaB kinase signalsome activation and IkappaB proteolysis. J Biol Chem. 1999; 274(35):24525-24632. (Biology). View Reference
  2. Page S, Fischer C, Baumgartner B, et al. 4-Hydroxynonenal prevents NF-kappaB activation and tumor necrosis factor expression by inhibiting IkappaB phosphorylation and subsequent proteolysis. J Biol Chem. 1999; 274(17):11611-11618. (Biology). View Reference
  3. Simeonidis S, Liang S, Chen G, Thanos D. Cloning and functional characterization of mouse IkappaBepsilon. Proc Natl Acad Sci U S A. 1997; 94(26):14327-14377. (Biology). View Reference
  4. Whiteside ST, Epinat JC, Rice NR, Israel A. I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity. EMBO J. 1997; 16(6):1413-1426. (Biology). View Reference
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611408 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.