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Western blot analysis of β-Enolase on a rat muscle lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti- β-Enolase antibody.


BD Transduction Laboratories™ Purified Mouse Anti- β-Enolase

Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
Western blot: Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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Enolases are glycolytic enzymes that interconvert 2-phosphoglycerate to phosphoenolpyruvate. They exist as three structurally related but genetically distinct isoforms. The active form of the enzyme is a homodimer formed from one of three subunits (α, β, and γ) that are encoded by distinct genes. The expression of each enolase gene is regulated in a tissue- and developmental- specific manner. While α-enolase has been reported to be expressed in nearly all embryonic and adult tissues, a developmental switch occurs between α and γ isoforms in cells of neuronal origin and between α- and β-enolase in developing skeletal muscle and heart. Early in embryogenesis, β-enolase reportedly is expressed at low levels in skeletal primary fibers. Increases in the expression of β-enolase are detected at the fetal stage of development and after birth. Levels of β-enolase are further increased in adult fast-twitch fibers and with terminal differentiation. In addition, β-enolase expression is regulated during hypoxia via the modulation of Sp1/Sp3 transcription factors levels. Thus, β-enolase is a muscle specific enolase that is thought to be essential for proper development and differentiation of myocytes.
This antibody is routinely tested by western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.
Development References (3)
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Discher DJ, Bishopric NH, Wu X, Peterson CA, Webster KA. Hypoxia regulates beta-enolase and pyruvate kinase-M promoters by modulating Sp1/Sp3 binding to a conserved GC element. J Biol Chem. 1998; 273(40):26087-26093. (Biology). View Reference
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Feo S, Antona V, Barbieri G, Passantino R, Cali L, Giallongo A. Transcription of the human beta enolase gene (ENO-3) is regulated by an intronic muscle-specific enhancer that binds myocyte-specific enhancer factor 2 proteins and ubiquitous G-rich-box binding factors. Mol Biol Cell. 1995; 15(11):5991-6002. (Biology). View Reference
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Giallongo A, Venturella S, Oliva D, Barbieri G, Rubino P, Feo S. Structural features of the human gene for muscle-specific enolase. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA. Eur J Biochem. 1993; 214(2):367-374. (Biology). View Reference
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