Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Companion Products
The 16E5 monoclonal antibody specifically recognizes Trem-like 4 (Treml4) which is also known as Trem-like transcript 4 protein (TLT-4 or TLT4). Treml4 is a type I transmembrane glycoprotein protein that is encoded by Treml4 (Treml4 triggering receptor expressed on myeloid cells-like 4). Treml4 is comprised of a single IgV-like domain in its extracellular region that is followed by a transmembrane segment and a short cytoplasmic tail. Treml4 is expressed on splenic macrophages and dendritic cells (DC), particularly CD8+ dendritic cells. Treml4 can associate with DAP12 and regulate immune responses. It reportedly binds to B7-H3 and to apoptotic or necrotic cells.
Development References (3)
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Hemmi H, Idoyaga J, Suda K, et al. A new triggering receptor expressed on myeloid cells (Trem) family member, Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linked marker for subsets of mouse macrophages and dendritic cells.. J Immunol. 2009; 182(3):1278-86. (Immunogen: Flow cytometry, Western blot). View Reference
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Hemmi H, Zaidi N, Wang B, et al. Treml4, an Ig superfamily member, mediates presentation of several antigens to T cells in vivo, including protective immunity to HER2 protein.. J Immunol. 2012; 188(3):1147-55. (Clone-specific: Flow cytometry). View Reference
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Pek RH, Yuan X, Rietzschel N, et al. Hemozoin produced by mammals confers heme tolerance.. Elife. 2019; 8:e49503. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.