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      RY586 Mouse Anti-Mouse SLAMF9
      Product Details
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      BD OptiBuild™
      CD2F-10; Cd2f10; SLAM family member 9; Slamf9
      Mouse (Tested in Development)
      Mouse C57BL/6 IgG1, κ
      Mouse SLAMF9 Fusion Protein
      Flow cytometry (Qualified)
      0.2 mg/ml
      98365
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Researchers should determine the optimal concentration of this reagent for their individual applications.
      2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. CF™ is a trademark of Biotium, Inc.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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      756080 Rev. 1
      Antibody Details
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      M349

      The M349 monoclonal antibody specifically recognizes mouse Signaling lymphocyte activation molecule family member 9 that is also known as SLAM family member 9 (SLAMF9) and CD2 antigen family member 10 (Cd2f10, CD2F-10). SLAMF9 is a type I transmembrane glycoprotein that is encoded by Slamf9 which belongs to the SLAM (CD150)/CD2 subset of the Ig superfamily. SLAMF9 is comprised of an N-terminal extracellular domain that contains an IgV-type domain followed by a IgC2-type domain and a transmembrane region. SLAMF9 has a short lysine- and arginine- rich cytoplasmic domain that lacks Immunoreceptor Tyrosine-based Switch Motifs (ITSMs) characteristic of other SLAM/CD2 subset members. SLAMF9 is variably expressed on plasmacytoid dendritic cells, peritoneal B1 cells and F4/80-low macrophages. SLAMF9 may play a role in the induction of pro-inflammatory cytokines and bacterial clearance.

      756080 Rev. 1
      Format Details
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      RY586
      The BD Horizon RealYellow™ 586 (RY586) Dye is part of the BD family of yellow-green dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 565-nm and an emission maximum (Em Max) at 586-nm. Driven by BD innovation, RY586 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm Blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY586 can be used as an alternative to PE and we recommend using an optical filter centered near 586-nm (eg, a 586/15-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE. Compared to PE, RY586 is similar in brightness, minimal spillover into Blue detectors, and increased spillover into the 610/20-nm (PE-CF594) detector. Please ensure that your instrument configuration (lasers and optical filters) is appropriate for this dye.
      altImg
      RY586
      Yellow-Green 561 nm
      564 nm
      586 nm
      756080 Rev.1
      Citations & References
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      View product citations for antibody "756080" on CiteAb

      Development References (5)

      1. Cannons JL, Tangye SG, Schwartzberg PL. SLAM family receptors and SAP adaptors in immunity. Annu Rev Immunol. 2011; 29:665-705. (Biology). View Reference
      2. Dollt C, Michel J, Kloss L, et al. The novel immunoglobulin super family receptor SLAMF9 identified in TAM of murine and human melanoma influences pro-inflammatory cytokine secretion and migration.. Cell Death Dis. 2018; 9(10):939. (Biology). View Reference
      3. Mikulin JA, Bates BL, Wilson TJ. A simplified method for separating renal MPCs using SLAMF9.. Cytometry A. 2021; 99(12):1209-1217. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
      4. Sever L, Radomir L, Stirm K, et al. SLAMF9 regulates pDC homeostasis and function in health and disease.. Proc Natl Acad Sci U S A. 2019; 116(33):16489-16496. (Biology). View Reference
      5. Wilson TJ, Clare S, Mikulin J, et al. Signalling lymphocyte activation molecule family member 9 is found on select subsets of antigen-presenting cells and promotes resistance to Salmonella infection.. Immunology. 2020; 159(4):393-403. (Immunogen: Flow cytometry). View Reference
      View All (5) View Less
      756080 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.