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RB670 Rat Anti-Mouse CD117 (c-Kit)
Product Details
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BD OptiBuild™
CD117; c-KIT; Kit; SCFR; Steel Factor Receptor
Mouse (Tested in Development)
Rat Wistar IgG2b, κ
Mouse IL-3-dependent Mast Cells
Flow cytometry (Qualified)
0.2 mg/ml
16590
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  3. For U.S. patents that may apply, see bd.com/patents.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  7. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  8. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  9. An isotype control should be used at the same concentration as the antibody of interest.
  10. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
  11. Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
  12. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
771219 Rev. 1
Antibody Details
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ACK2

The ACK2 monoclonal antibody specifically binds to CD117, which is also known as Proto-oncogene c-Kit (c-kit), Stem Cell Factor Receptor (SCFR), and Mast/stem cell growth factor receptor. This ~145 kDa type I transmembrane glycoprotein is encoded by Kit (KIT proto-oncogene receptor tyrosine kinase) which belongs to the receptor tyrosine kinase family (RTK) within the immunoglobulin superfamily (IgSF). CD117 (c-kit) serves as a receptor for c-Kit ligand (aka, stem cell factor or SCF). This receptor is expressed on multipotent hematopoietic stem cells and progenitors committed to myeloid and/or erythroid lineages, and precursors of B and T lymphocytes. CD117 (c-kit)-mediated signaling plays an important role in the activation, growth, proliferation, differentiation, and survival of cells in sustaining hematopoiesis. It is also involved in the development of mast cells and melanocytes. The specificity and functional activity of the ACK2 antibody is like that of another CD117 (c-kit)-specific antibody, ACK45. ACK2 is reported to block CD117 (c-kit) signaling in vitro and in vivo.

771219 Rev. 1
Format Details
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RB670
The BD Horizon RealBlue™ 670 (RB670) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 492 nm and an emission maximum (Em Max) at 670 nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB670 can be used on both spectral and conventional cytometers and is designed to be primarily excited by the Blue laser (488-nm). For conventional instruments equipped with only a Blue laser (488-nm), RB670 can be used as an alternative to PE-Cy5 and we recommend using an optical filter centered near 670-nm (eg, a 670/30-nm bandpass filter). For conventional and spectral instruments equipped with both a Blue (488-nm) and Yellow-Green (561-nm) laser and appropriate detectors, it can be used in conjunction with PE-Cy5.
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RB670
Blue 488 nm
492 nm
670 nm
771219 Rev.1
Citations & References
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View product citations for antibody "771219" on CiteAb

Development References (6)

  1. Czechowicz A, Kraft D, Weissman IL, Bhattacharya D. Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches.. Science. 2007; 318(5854):1296-9. (Clone-specific: Flow cytometry, Functional assay, Inhibition, In vivo exacerbation). View Reference
  2. Géraud C, Koch PS, Zierow J, et al. GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis.. J Clin Invest. 2017; 127(3):1099-1114. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  3. Hozumi K, Kobori A, Sato T, et al. Pro-T cells in fetal thymus express c-kit and RAG-2 but do not rearrange the gene encoding the T cell receptor beta chain.. Eur J Immunol. 1994; 24(6):1339-44. (Clone-specific: Functional assay, Inhibition). View Reference
  4. Nishikawa S, Kusakabe M, Yoshinaga K, et al. In utero manipulation of coat color formation by a monoclonal anti-c-kit antibody: two distinct waves of c-kit-dependency during melanocyte development. EMBO J. 1991; 10(8):2111-2118. (Immunogen: Functional assay, Immunohistochemistry, Inhibition, In vivo exacerbation). View Reference
  5. Ogawa M, Matsuzaki Y, Nishikawa S, et al. Expression and function of c-kit in hemopoietic progenitor cells. J Exp Med. 1991; 174(1):63-71. (Immunogen: Functional assay, Inhibition, In vivo exacerbation). View Reference
  6. Rico-Vargas SA, Weiskopf B, Nishikawa S, Osmond DG. c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody. J Immunol. 1994; 152(6):2845-2852. (Clone-specific: Immunofluorescence, In vivo exacerbation). View Reference
View All (6) View Less
771219 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.