.png){kind=spacer}
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Researchers should determine the optimal concentration of this reagent for their individual applications.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
Companion Products
.png?imwidth=320)
The H132 monoclonal antibody specifically recognizes the variable beta 13.2 region of the β subunit of the human αβ T cell receptor for antigen (TCR Vβ13.2). TCR Vβ13.2 is expressed on subsets of TCR αβ positive thymocytes and peripheral CD4+ and CD8+ T cells. The H132 antibody is useful for multiparameter analyses designed to study the nature of TCR Vβ13.2-positive cells including normal T cells as well as T cell clones, hybridomas, or tumors. It is also useful for analyzing TCR Vβ repertoires expressed by T cell populations collected from blood, tissues or other sources in health and disease models including inflammation, autoimmunity, responses to superantigens, tumors, and infectious diseases including HIV infection. The H132 antibody can reportedly stimulate the proliferation of TCR Vβ13.2-positive T cells. The H132 antibody recognizes human TCR Vβ13.2 subfamily members but does not react with other human TCR Vβ13 family members including TCR Vβ13.1, Vβ13.3, Vβ13.5 and Vβ13.6 subfamily members.
Development References (5)
-
Choi YW, Kotzin B, Lafferty J, et al. A method for production of antibodies to human T-cell receptor beta-chain variable regions.. Proc Natl Acad Sci USA. 1991; 88(19):8357-61. (Immunogen: Flow cytometry, Functional assay, Stimulation). View Reference
-
Dong T, Stewart-Jones G, Chen N, et al. HIV-specific cytotoxic T cells from long-term survivors select a unique T cell receptor.. J Exp Med. 2004; 200(12):1547-57. (Biology). View Reference
-
Mongkolsapaya J, Jaye A, Callan MF, Magnusen AF, McMichael AJ, Whittle HC. Antigen-specific expansion of cytotoxic T lymphocytes in acute measles virus infection.. J Virol. 1999; 73(1):67-71. (Clone-specific: Flow cytometry). View Reference
-
Moss P, Gillespie G, Frodsham P, Bell J, Reyburn H. Clonal populations of CD4+ and CD8+ T cells in patients with multiple myeloma and paraproteinemia. Blood. 1996; 87(8):3296-3306. (Clone-specific: Flow cytometry). View Reference
-
Salameire D, Solly F, Fabre B, et al. Accurate detection of the tumor clone in peripheral T-cell lymphoma biopsies by flow cytometric analysis of TCR-Vβ repertoire.. Mod Pathol. 2012; 25(9):1246-57. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.