Preparation And Storage
Recommended Assay Procedures
Because of the low density expression of 4-1BBL on activated B lymphocytes, we recommend the use of a bright second-step reagent, such as PE-conjugated anti-rat Ig polyclonal antibody (Cat. No. 550767). Other reported applications include the blocking of the functional interaction of 4-1BBL with 4-1BB.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Companion Products
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The TKS-1 monoclonal antibody specifically recognizes 4-1BB Ligand (4-1BBL), a 97 kDa member of the NGF/TNF superfamily which is present on antigen-presenting cells and activated B lymphocytes. 4-1BBL interacts with 4-1BB (CDw137), found predominantly on activated T lymphocytes. This ligand-receptor pair is grouped with pairs such as CD27/CD70, CD30/CD153, OX-40/OX-40L, CD80/CD28, and CD86/CD28, which costimulate T-cell proliferation and cytokine production. The interaction of 4-1BB with 4-1BBL may be reciprocally costimulatory in that 4-1BBL may deliver proliferative signals to B lymphocytes activated by anti-IgM. The TKS-1 mAb stains B cells activated for three days with anti-IgM polyclonal antibody, consistent with an earlier report that soluble 4-1BB-alkaline phosphatase fusion protein binds to anti-IgM-activated B cells. Furthermore, 4-1BBL mRNA has been detected in activated T lymphocytes and activated macrophages. The TKS-1 mAb has been reported to inhibit the binding of soluble 4-1BB to 4-1BBL transfectants and block the in vitro costimulation of T-lymphocyte proliferation by 4-1BBL transfectants.
Development References (5)
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Akiba H, Miyahira Y, Atsuta M. Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis. J Exp Med. 2000; 191(2):375-382. (Immunogen: Blocking). View Reference
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DeBenedette MA, Shahinian A, Mak TW, Watts TH. Costimulation of CD28- T lymphocytes by 4-1BB ligand. J Immunol. 1997; 158(2):551-559. (Biology). View Reference
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Goodwin RG, Din WS, Davis-Smith T, et al. Molecular cloning of a ligand for the inducible T cell gene 4-1BB: a member of an emerging family of cytokines with homology to tumor necrosis factor. Eur J Immunol. 1993 October; 23(10):2631-2641. (Biology). View Reference
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Pollok KE, Kim SH, Kwon BS. Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4. Eur J Immunol. 1995; 25(2):488-494. (Biology). View Reference
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Pollok KE, Kim YJ, Hurtado J, Zhou Z, Kim KK, Kwon BS. 4-1BB T-cell antigen binds to mature B cells and macrophages, and costimulates anti-mu-primed splenic B cells. Eur J Immunol. 1994, February; 24(2):367-374. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
