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Purified Mouse Anti-Rat Erythroid Cells
Purified Mouse Anti-Rat Erythroid Cells
Three-color analysis of erythroid cells in the rat bone marrow. Cells flushed from LEW femoral bone marrow were simultaneously stained with PE-conjugated anti-rat CD71 mAb OX-26 (Cat. no. 554891), biotin-conjugated anti-rat CD45 mAb OX-1 (Cat. no. 554876), and purified mAb HIS49 (Cat. No. 550961), followed by Streptavidin-APC (Cat. no. 554067) and FITC-conjugated anti-mouse IgM mAb R6-60.2 (Cat. no. 553408). The two-color contour plot of CD45 vs. CD71 expression is displayed in Panel A.  The remaining panels display histograms of HIS49 antigen expression on the gated subpopulations: B, CD45+ CD71+ bright immature erythroid cells; C, CD45- CD71+ maturing erythroid cells; D, CD45- CD71- erythrocytes; E, CD45+ CD71+ hematopoietic progenitors; and F, CD45+ CD71- leukocytes. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system with data analysis by FlowJo software (TreeStar).
Three-color analysis of erythroid cells in the rat bone marrow. Cells flushed from LEW femoral bone marrow were simultaneously stained with PE-conjugated anti-rat CD71 mAb OX-26 (Cat. no. 554891), biotin-conjugated anti-rat CD45 mAb OX-1 (Cat. no. 554876), and purified mAb HIS49 (Cat. No. 550961), followed by Streptavidin-APC (Cat. no. 554067) and FITC-conjugated anti-mouse IgM mAb R6-60.2 (Cat. no. 553408). The two-color contour plot of CD45 vs. CD71 expression is displayed in Panel A.  The remaining panels display histograms of HIS49 antigen expression on the gated subpopulations: B, CD45+ CD71+ bright immature erythroid cells; C, CD45- CD71+ maturing erythroid cells; D, CD45- CD71- erythrocytes; E, CD45+ CD71+ hematopoietic progenitors; and F, CD45+ CD71- leukocytes. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system with data analysis by FlowJo software (TreeStar).
Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgM, κ
Rat bone marrow cells
Flow cytometry (Routinely Tested), Immunofluorescence, Immunohistochemistry-frozen (Reported)
0.5 mg/ml
AB_393978
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

The HIS49 antibody can cause aggregation of erythrocytes, which results in depletion of erythrocytes in a concentration-dependent manner.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550961 Rev. 6
Antibody Details
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HIS49

The HIS49 antibody reacts with an antigen found on erythrocytes and erythroid progenitors, but not on granulocytes, monocytes, or lymphocytes.

550961 Rev. 6
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
550961 Rev.6
Citations & References
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View product citations for antibody "550961" on CiteAb

Development References (1)

  1. Hermans MH, Opstelten D. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem. 1991; 39(12):1627-1634. (Immunogen: Immunofluorescence, Immunohistochemistry). View Reference
550961 Rev. 6

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.