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      PE Rat Anti-Mouse NK-T/NK Cell Antigen
      PE Rat Anti-Mouse NK-T/NK Cell Antigen
      Expression of  the NK-T/NK Cell Antigen on mouse hepatic mononuclear cells. C57BL/6 intrahepatic lymphocytes were stained with FITC-conjugated anti-mouse CD3e (Cat. No. 553061/553062), PE-conjugated U5A2-13, and biotinylated anti-mouse NK-1.1 (Cat. No. 553163) mAbs in the presence of Mouse Fc Block™ (Cat. No. 553141/553142), followed by Streptavidin-APC (Cat. No. 554067). The following four subpopulations of viable lymphocytes (Via-Probe™, Cat. No. 555816/555815) were selected for data analysis: NK1.1+ CD3-, NK1.1+ CD3+, NK1,1- CD3-, NK1.1- CD3+ (R3, R4, R6, and R5, respectively, in panel A). The expression of the NK-T/NK Cell Antigen is shown as filled histograms, with isotype control as empty histograms, in Panels R3, R4, R6, and R5 displaying the respective subpopulations. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      PE Rat Anti-Mouse NK-T/NK Cell Antigen
      Expression of  the NK-T/NK Cell Antigen on mouse hepatic mononuclear cells. C57BL/6 intrahepatic lymphocytes were stained with FITC-conjugated anti-mouse CD3e (Cat. No. 553061/553062), PE-conjugated U5A2-13, and biotinylated anti-mouse NK-1.1 (Cat. No. 553163) mAbs in the presence of Mouse Fc Block™ (Cat. No. 553141/553142), followed by Streptavidin-APC (Cat. No. 554067). The following four subpopulations of viable lymphocytes (Via-Probe™, Cat. No. 555816/555815) were selected for data analysis: NK1.1+ CD3-, NK1.1+ CD3+, NK1,1- CD3-, NK1.1- CD3+ (R3, R4, R6, and R5, respectively, in panel A). The expression of the NK-T/NK Cell Antigen is shown as filled histograms, with isotype control as empty histograms, in Panels R3, R4, R6, and R5 displaying the respective subpopulations. Flow cytometry was performed on a FACSCalibur™ (BDIS, San Jose, CA).
      Product Details
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      BD Pharmingen™
      ICAM-1
      Mouse (QC Testing)
      Rat F344, also known as Fischer, CDF IgG2a, κ
      BALB/c nu/nu mouse lymphoma line tMK-2U
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      AB_393552
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      For optimal immunofluorescent staining, we recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32 2.4G2 mAb, Cat. No. 553141/553142).

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550082 Rev. 7
      Antibody Details
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      U5A2-13

      The U5A2-13 mAb recognizes an antigen expressed on most-NKT cells and 55% of NK cells in the liver of various mouse strains, including both NK1.1+ (e.g., C57BL/6) and NK1.1- (e.g., BALB/c, C3H/He, MRL/lpr) strains. Abundant IL-4 and IFN-γ are produced by U5A2-13 Ag+ TCR αβ+ cells upon CD3 cross-linking, but not by U5A2-13 Ag- TCR αβ+ cells in both NK1.1+ and NK1.1- strains. In C57BL/6 mice (NK1.1+ strain), 80%of U5A2-13 Ag+ T cells co-express the NK1.1 molecule. When staining hepatic mononuclear cells with U5A2-13 mAb, the fluorescence intensity detected on on CD3- cells is lower than on CD3+ cells in BALB/c and C57BL/6 mice. Immunoprecipitation studies have shown that the U5A2-13 antigen comprises three proteins of 65 kDa, 34 kDa, and 31 kDa. This antibody clone U5A2-13 was originally established for mouse NKT cells, but recent studies showed that it recognized a novel conformational epitope of ICAM-1.

      550082 Rev. 7
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      550082 Rev.7
      Citations & References
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      Development References (4)

      1. Kato K, Ikarashi Y, Sugahara T, et al. U5A2-13, an antigen originally found on mouse NK-like T cells, is an early inducible cell surface antigen during lymphoid activation.. Cell Immunol. 2003; 221(1):27-36. (Clone-specific). View Reference
      2. Maruoka H, Ikarashi Y, Shinohara K, et al. A novel monoclonal antibody permitting recognition of NKT cells in various mouse strains. Biochem Biophys Res Commun. 1998; 242(2):413-418. (Immunogen). View Reference
      3. Shimizu A, Sasaki H, Aoyagi K, et al. The mouse natural killer T cell-associated antigen recognized by U5A2-13 monoclonal antibody is intercellular adhesion molecule-1.. Immunol Lett. 2004; 92(3):227-235. (Clone-specific). View Reference
      4. Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference
      View All (4) View Less
      550082 Rev. 7

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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