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      PE Rat Anti-Mouse CD226
      PE Rat Anti-Mouse CD226
      Multicolor flow cytometric analysis of CD226 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Hamster Anti-Mouse CD3e (Cat. No. 562600; Top Plots) and APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093; Bottom Plots) antibodies, and either PE Rat IgG2b κ Isotype Control (Cat. No. 553989; Left Plots) or PE Rat Anti-Mouse CD226 antibody (Cat. No. 565663; Right Plots) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD226 (or Ig Isotype control staining) versus CD3e or CD8a were derived from gated events with the forward and side light-scattering characteristics of viable 7-AAD-negative leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
      PE Rat Anti-Mouse CD226
      Multicolor flow cytometric analysis of CD226 expression on mouse splenocytes. BALB/c mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with BD Horizon™ BV421 Hamster Anti-Mouse CD3e (Cat. No. 562600; Top Plots) and APC Rat Anti-Mouse CD8a (Cat. No. 553035/561093; Bottom Plots) antibodies, and either PE Rat IgG2b κ Isotype Control (Cat. No. 553989; Left Plots) or PE Rat Anti-Mouse CD226 antibody (Cat. No. 565663; Right Plots) at 0.5 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. Two-color flow cytometric contour plots showing the correlated expression of CD226 (or Ig Isotype control staining) versus CD3e or CD8a were derived from gated events with the forward and side light-scattering characteristics of viable 7-AAD-negative leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
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      BD Pharmingen™
      DNAM-1; DNAM1; PTA-1; TLiSA1
      Mouse (QC Testing)
      Rat IgG2b, κ
      Mouse Th1 Cells
      Flow cytometry (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      565663 Rev. 1
      Antibody Details
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      10E5

      The 10E5 monoclonal antibody specifically binds to CD226 which is also known as, Platelet and T-cell activation antigen 1 (Pta1), T lineage-specific activation antigen 1 (TLiSA1), or DNAX accessory molecule-1 (DNAM-1). CD226 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD226 serves as an adhesion molecule that is primarily expressed on T lymphocytes. Naïve CD8+ T cells express high levels of CD226 whereas it is differentially expressed on CD4+ T cells. In certain model systems, long term-cloned Th1 cells can express high CD226 levels when compared with the downregulated CD226 expression exhibited on cloned Th2 and Th0 cells. CD226 expression is also detectable on subsets of CD11b+ macrophages and NK cells. Adhesive interactions between CD226 and its ligands, CD112 and CD155, can result in cell signaling events that promote innate and adaptive immune responses, including the differentiation and survival of cytotoxic cells.

      565663 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      565663 Rev.1
      Citations & References
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      View product citations for antibody "565663" on CiteAb

      Development References (4)

      1. Dardalhon V, Schubart AS, Reddy J, et al. CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. Immunology. 2005; 175(3):1558-1565. (Immunogen: Flow cytometry, Inhibition, In vivo exacerbation). View Reference
      2. Seth S, Georgoudaki AM, Chambers BJ et al. Heterogeneous expression of the adhesion receptor CD226 on murine NK and T cells and its function in NK-mediated killing of immature dendritic cells. J Leukoc Biol. 2009; 86(1):91-101. (Biology). View Reference
      3. Tahara-Hanaoka S, Shibuya K, Kai H, et al. Blood. 2006; 107(4):491-496. (Biology). View Reference
      4. Tahara-Hanaoka S, Shibuya K, Onoda Y et al. Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112). Int Immunol. 2006; 16(4):533-538. (Biology). View Reference
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      565663 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.