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Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
Companion Products
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The 4E2 monoclonal antibody specifically binds to CD121b which is also known as the IL-1 receptor type II (IL-1RII, IL-1R2) or IL-1 receptor beta (IL-1R-beta, IL-1Rβ). CD121b is encoded by the Il1r2 gene and presents as a 68 kDa type I transmembrane glycoprotein. It is expressed on a variety of cell types including B cells, neutrophils, monocytes, and bone marrow progenitor cells. A soluble form of CD121b is produced by proteolysis especially after cellular activation. Unlike the IL-1 type I receptor (CD121a), CD121b has a short cytoplasmic domain and does not mediate IL-1 signaling. CD121b may function in a membrane or soluble form as a decoy receptor that can bind IL-1 and reduce its effective concentration and biological activity. The 4E2 antibody blocks IL-1 binding to the IL-1 receptor type II.
Development References (7)
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Colotta F, Re F, Muzio M, et al. Interleukin-1 type II receptor: a decoy target for IL-1 that is regulated by IL-4. Science. 1993; 261(5120):472-475. (Biology). View Reference
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Cumberbatch M, Dearman RJ, Kimber I. Characteristics and regulation of the expression on interleukin 1 receptors by murine Langerhans cells and keratinocytes. Arch Dermatol Res. 1998; 290(12):688-695. (Clone-specific: Flow cytometry). View Reference
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Hestdal K, Ruscetti FW, Chizzonite R, et al. Interleukin-1 (IL-1) directly and indirectly promotes hematopoietic cell growth through type I IL-1 receptor. Blood. 1994; 84(1):125-132. (Clone-specific). View Reference
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Hunter CA, Chizzonite R, Remington JS. IL-1 beta is required for IL-12 to induce production of IFN-gamma by NK cells. A role for IL-1 beta in the T cell-independent mechanism of resistance against intracellular pathogens. J Immunol. 1995; 155(9):4347-4354. (Clone-specific: Neutralization). View Reference
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McMahan CJ, Slack JL, Mosley B, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J. 1991; 10(10):2821-2832. (Biology). View Reference
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Oldenburg HS, Pruitt JH, Lazarus DD, et al. Interleukin 1 binding to its type I, but not type II receptor, modulates the in vivo acute phase response. Cytokine. 1995; 7(6):510-516. (Clone-specific). View Reference
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Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.