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Flow cytometric analysis of Viperin expression on LPS-stimulated lymphocytes. Human peripheral blood mononuclear cells were cultured overnight either without (Unstimulated; Left Panel) or with (LPS-Stimulated; Right Panel) lipopolysaccharide (1 μg/ml). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were stained with PE Mouse IgG2a, κ Isotype Control (Cat. No.554648; dashed line histograms) or PE Mouse Anti-Viperin antibody (Cat. No. 565196; solid line histograms). Histograms showing Viperin (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
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BD Pharmingen™ PE Mouse Anti-Viperin
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Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
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The MaP.VIP monoclonal antibody specifically binds to Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible (Viperin), which is also known as Radical S-adenosyl methionine domain-containing protein 2 (Rsad2). Viperin is a 43 kDa protein that is localized to the endoplasmic reticulum and lipid droplets. It functions in protective responses to a number of different DNA and RNA viruses. Viperin expression is induced in cells by different agents such as, Type I interferons, Interferon-γ (IFN-γ), DNA and RNA viral proteins, Polyinosinic:polycytidylic acid, and lipopolysaccharide. Various cell types can be induced to express Viperin including T and B lymphocytes, macrophages, granulocytes, dendritic cells, fibroblasts, endothelial cells, and epithelial cells.

Development References (9)
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Hinson ER, Cresswell P. The N-terminal amphipathic alpha-helix of viperin mediates localization to the cytosolic face of the endoplasmic reticulum and inhibits protein secretion. J Biol Chem. 2009; 284(7):4705-4712. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Western blot). View Reference
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Hinson ER, Cresswell P. The antiviral protein, viperin, localizes to lipid droplets via its N-terminal amphipathic alpha-helix. Proc Natl Acad Sci U S A. 2009; 106(48):20452-20457. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
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Hinson ER, Joshi NS, Chen JH, et al. Viperin is highly induced in neutrophils and macrophages during acute and chronic lymphocytic choriomeningitis virus infection. J Immunol. 2010; 184(10):5723-5731. (Clone-specific: Electron microscopy, Flow cytometry, Immunocytochemistry (cytospins)). View Reference
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Mattijssen S, Pruijn GJ. Viperin, a key player in the antiviral response. Microbes Infect. 2012; 14(5):419-426. (Biology). View Reference
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Qiu LQ, Cresswell P, Chin KC. Viperin is required for optimal Th2 responses and T-cell receptor-mediated activation of NF-kappaB and AP-1. Blood. 2009; 113(15):3520-3529. (Biology). View Reference
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Seo JY, Cresswell P. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. PLoS ONE. 2013; 9(8):e1003497. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
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Seo JY, Yaneva R, Hinson ER, Cresswell P. Human cytomegalovirus directly induces the antiviral protein viperin to enhance infectivity. Science. 2011; 332(6033):1093-1097. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Immunoprecipitation). View Reference
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Szretter KJ, Brien JD, Thackray LB, Virgin HW, Cresswell P, Diamond MS. The interferon-inducible gene viperin restricts West Nile virus pathogenesis. J Virol. 2011; 85(22):11557-11566. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
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Wang X, Hinson ER, Cresswell P. The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell. 2007; 2(2):96-105. (Immunogen: Fluorescence microscopy, Immunofluorescence). View Reference
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