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PE Mouse Anti-Viperin
PE Mouse Anti-Viperin
Flow cytometric analysis of Viperin expression on LPS-stimulated lymphocytes. Human peripheral blood mononuclear cells were cultured overnight either without (Unstimulated; Left Panel) or with (LPS-Stimulated; Right Panel) lipopolysaccharide (1 μg/ml). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were stained with PE Mouse IgG2a, κ Isotype Control (Cat. No.554648; dashed line histograms) or PE Mouse Anti-Viperin antibody (Cat. No. 565196; solid line histograms). Histograms showing Viperin (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Flow cytometric analysis of Viperin expression on LPS-stimulated lymphocytes. Human peripheral blood mononuclear cells were cultured overnight either without (Unstimulated; Left Panel) or with (LPS-Stimulated; Right Panel) lipopolysaccharide (1 μg/ml). The cells were washed, and then fixed and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Solution Kit (Cat. No. 554714). Cells were stained with PE Mouse IgG2a, κ Isotype Control (Cat. No.554648; dashed line histograms) or PE Mouse Anti-Viperin antibody (Cat. No. 565196; solid line histograms). Histograms showing Viperin (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
Product Details
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BD Pharmingen™
Rsad2; Vig1; cig5
Human (QC Testing), Mouse (Tested in Development), Rat (Predicted)
Mouse IgG2a, κ
Mouse Viperin Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739106
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
565196 Rev. 1
Antibody Details
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MaP.VIP

The MaP.VIP monoclonal antibody specifically binds to Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible (Viperin), which is also known as Radical S-adenosyl methionine domain-containing protein 2 (Rsad2). Viperin is a 43 kDa protein that is localized to the endoplasmic reticulum and lipid droplets. It functions in protective responses to a number of different DNA and RNA viruses. Viperin expression is induced in cells by different agents such as, Type I interferons, Interferon-γ (IFN-γ), DNA and RNA viral proteins, Polyinosinic:polycytidylic acid, and lipopolysaccharide.  Various cell types can be induced to express Viperin including T and B lymphocytes, macrophages, granulocytes, dendritic cells, fibroblasts, endothelial cells, and epithelial cells.

565196 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
565196 Rev.1
Citations & References
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View product citations for antibody "565196" on CiteAb

Development References (9)

  1. Hinson ER, Cresswell P. The N-terminal amphipathic alpha-helix of viperin mediates localization to the cytosolic face of the endoplasmic reticulum and inhibits protein secretion. J Biol Chem. 2009; 284(7):4705-4712. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Western blot). View Reference
  2. Hinson ER, Cresswell P. The antiviral protein, viperin, localizes to lipid droplets via its N-terminal amphipathic alpha-helix. Proc Natl Acad Sci U S A. 2009; 106(48):20452-20457. (Clone-specific: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
  3. Hinson ER, Joshi NS, Chen JH, et al. Viperin is highly induced in neutrophils and macrophages during acute and chronic lymphocytic choriomeningitis virus infection. J Immunol. 2010; 184(10):5723-5731. (Clone-specific: Electron microscopy, Flow cytometry, Immunocytochemistry (cytospins)). View Reference
  4. Mattijssen S, Pruijn GJ. Viperin, a key player in the antiviral response. Microbes Infect. 2012; 14(5):419-426. (Biology). View Reference
  5. Qiu LQ, Cresswell P, Chin KC. Viperin is required for optimal Th2 responses and T-cell receptor-mediated activation of NF-kappaB and AP-1. Blood. 2009; 113(15):3520-3529. (Biology). View Reference
  6. Seo JY, Cresswell P. Viperin regulates cellular lipid metabolism during human cytomegalovirus infection. PLoS ONE. 2013; 9(8):e1003497. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
  7. Seo JY, Yaneva R, Hinson ER, Cresswell P. Human cytomegalovirus directly induces the antiviral protein viperin to enhance infectivity. Science. 2011; 332(6033):1093-1097. (Clone-specific: Electron microscopy, Fluorescence microscopy, Immunocytochemistry (cytospins), Immunofluorescence, Immunoprecipitation). View Reference
  8. Szretter KJ, Brien JD, Thackray LB, Virgin HW, Cresswell P, Diamond MS. The interferon-inducible gene viperin restricts West Nile virus pathogenesis. J Virol. 2011; 85(22):11557-11566. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
  9. Wang X, Hinson ER, Cresswell P. The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell. 2007; 2(2):96-105. (Immunogen: Fluorescence microscopy, Immunofluorescence). View Reference
View All (9) View Less
565196 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.