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      PE Mouse Anti-Mouse Stat6 (pY641)
      PE Mouse Anti-Mouse Stat6 (pY641)
      Analysis of Stat6 (pY641) in activated mouse B lymphocytes. Mouse M12 B lymphoma cells were either stimulated with mouse IL-4 (Cat. No. 550067) at 37˚C for 15 minutes (filled histogram) or unstimulated (open histogram).  The cells were fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) at 37˚C for 10 minutes, permeabilized in BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes (or overnight at -20˚C), and stained with PE anti-mouse Stat6 (pY641) (Cat. No. 558252).  Flow cytometry was performed on a BD FACSCalibur™ instrument.
      PE Mouse Anti-Mouse Stat6 (pY641)
      Analysis of Stat6 (pY641) in activated mouse B lymphocytes. Mouse M12 B lymphoma cells were either stimulated with mouse IL-4 (Cat. No. 550067) at 37˚C for 15 minutes (filled histogram) or unstimulated (open histogram).  The cells were fixed with BD Phosflow™ Fix Buffer I (Cat. No. 557870) at 37˚C for 10 minutes, permeabilized in BD Phosflow™ Perm Buffer III (Cat. No. 558050) on ice for at least 30 minutes (or overnight at -20˚C), and stained with PE anti-mouse Stat6 (pY641) (Cat. No. 558252).  Flow cytometry was performed on a BD FACSCalibur™ instrument.
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      BD Phosflow™
      Mouse (QC Testing)
      Mouse IgG1, κ
      Phosphorylated Mouse STAT6 (Y641) Peptide
      Intracellular staining (flow cytometry) (Routinely Tested)
      20 µl
      AB_647233
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Recommended Assay Procedures

      Note: For flow cytometric analysis of human cells, PE Mouse Anti-Human Stat6 (pY641) (Cat. No. 612701) is recommended.

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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      558252 Rev. 3
      Antibody Details
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      J71-773.58.11

      STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone.  Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation.  Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes.  Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner.  Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors.  Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641).  The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain.  Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP.  While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta.

      The J71-773.58.11 antibody recognizes mouse Stat6 phosphorylated at Y641.

      558252 Rev. 3
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      558252 Rev.3
      Citations & References
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      View product citations for antibody "558252" on CiteAb

      Development References (8)

      1. Bromberg J, Darnell JE. The role of STATs in transcriptional control and their impact on cellular function. Oncogene. 2000; 19(21):2468-2473. (Biology). View Reference
      2. Daley JM, Brancato SK, Thomay AA, Reichner JS, Albina JE.. The phenotype of murine wound macrophages.. J Leukoc Biol. 2010; 87(1):59-67. (Biology). View Reference
      3. Dent AL, Hu-Li J, Paul WE, Staudt LM. T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6. Proc Natl Acad Sci U S A. 1998; 95(23):13823-13828. (Biology). View Reference
      4. Hale MB, Krutzik PO, Samra SS, Crane JM, Nolan GP.. Stage Dependent Aberrant Regulation of Cytokine-STAT Signaling in Murine Systemic Lupus Erythematosus. PLoS ONE. 2009; 4(8):e6756. (Biology). View Reference
      5. Heim MH. The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus. Eur J Clin Invest. 1996; 26(1):1-12. (Biology). View Reference
      6. Hou J, Schindler U, Henzel WJ, Ho TC, Brasseur M, McKnight SL. An interleukin-4-induced transcription factor: IL-4 Stat. Science. 1994; 265(5179):1701-1706. (Biology).
      7. Mikita T, Campbell D, Wu P, Williamson K, Schindler U. Requirements for interleukin-4-induced gene expression and functional characterization of Stat6. Mol Cell Biol. 1996; 16(10):5811-5820. (Biology).
      8. Quelle FW, Shimoda K, Thierfelder W, et al. Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis. Mol Cell Biol. 1995; 15(6):3336-3343. (Biology).
      View All (8) View Less
      558252 Rev. 3

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      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.