PE Mouse Anti-Human TSLP Receptor

BD Pharmingen™ PE Mouse Anti-Human TSLP Receptor

Name: PE Mouse Anti-Human TSLP Receptor
Brand: PE Mouse Anti-Human TSLP Receptor
SKU: 563149

Clone 1F11/TSLPR (also known as 1F11 and AB81_85.1F11) (RUO)

Flow cytometric analysis of human TSLP Receptor expression on human HEK-293 cells. HEK-293 cells cotransfected with constructs coding for human TSLPR and IL-7Rα/CD127 were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) that was mixed 1:1 with 1x Dulbecco's Phosphate Buffered Saline. The cells were then frozen at -80°C for subsequent testing. The frozen cells were thawed, their freezing media was removed, and the cells were washed. The cells were then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Panel) or PE Mouse Anti-Human TSLPR antibody (Cat. No. 563149; Right Panel). The flow cytometric fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. Histogram markers were set based on the Ig isotype control. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer System.

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Product Details

Brand: BD Pharmingen™

Alternative Name: CRL2; CRLF2; CRLF2Y; Cytokine receptor-like factor 2; IL-XR; TSLPR

Reactivity: Human (Tested in Development)

Isotype: Mouse IgG1, κ

Immunogen: Human TSLP Receptor Transfected Cell Line

Application: Flow cytometry (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_2738029

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Fixation Buffer RUO

Size 100 mL Cat No. 554655

PE Mouse IgG1, κ Isotype Control RUO

Size 0.1 mg Cat No. 554680

563149 Rev. 1

Antibody Details

1F11/TSLPR

The 1F11/TSLPR monoclonal antibody specifically binds to Thymic Stromal Lymphopoietin Receptor (TSLPR). TSLPR is a member of the hematopoietin receptor superfamily and is also known as Cytokine Receptor-like Factor 2 (CRL2, CRLF2Y, CRLF2). The functional TSLPR complex consists of two subunits, TSLPR and the alpha subunit of the Interleukin-7 Receptor (IL-7Rα). Analysis of the TSLPR reveals sequence similarity with the common cytokine receptor gamma chain (γc; CD132). Functional TSLPRs are expressed by epithelial cells and a variety of hematopoietic cell types, including thymocytes, T cells, B cells, natural killer T cells, monocytes, macrophages, basophils, and dendritic cells (DC). Recent studies indicate that TSLP can activate multiple STAT (Signal Transducer and Activator of Transcription) signaling proteins. TSLP enhances the maturation and viability of DC. It strongly induces DC expression of the CD40 and CD80 costimulatory molecules and chemokines, e.g., TARC (Thymus and activation-regulated chemokine; CCL17) that can attract Th2 effector cells. TSLP supports B cell development. TSLP costimulates the proliferation of naïve T cells in the presence of mature DC. TSLP is also able to increase the sensitivity of T cell receptor-activated CD4+ T cells to low doses of IL-2. In the presence of TSLP, the acute myeloid leukemia-derived cell line, MUTZ-3, shows induced growth and reduced apoptosis. CRLF2 deregulated gene expression is thought to be involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. The 1F11/TSLPR antibody is reportedly a neutralizing antibody.

563149 Rev. 1

Format Details

R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: PE

Excitation Source: Yellow-Green 488 nm, 532 nm, 561 nm

Excitation Max: 496 nm, 566 nm

Emission Max: 576 nm

563149 Rev.1

Citations & References

View product citations for antibody "563149" on CiteAb

Development References (10)

Arima K, Watanabe N, Hanabuchi S, Chang M, Sun SC, Liu YJ. Distinct signal codes generate dendritic cell functional plasticity. Sci Signal. 2010; 3(105):ra4. (Clone-specific: Neutralization). View Reference
Ito T, Liu YJ, Arima K. Cellular and molecular mechanisms of TSLP function in human allergic disorders--TSLP programs the "Th2 code" in dendritic cells. Allergol Int. 2012; 61(1):35-43. (Biology). View Reference
Lu N, Wang YH, Arima K, Hanabuchi S, Liu YJ. TSLP and IL-7 use two different mechanisms to regulate human CD4+ T cell homeostasis. J Exp Med. 2009; 206(10):2111-2119. (Biology). View Reference
Pedroza-Gonzalez A, Xu K, Wu TC, et al. Thymic stromal lymphopoietin fosters human breast tumor growth by promoting type 2 inflammation. J Exp Med. 2011; 208(3):479-490. (Clone-specific: Neutralization). View Reference
Quentmeier H, Drexler HG, Fleckenstein D, et al. Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation. Leukemia. 2001; 15(8):1286-1292. (Biology). View Reference
Reche PA, Soumelis V, Gorman DM, et al. Human thymic stromal lymphopoietin preferentially stimulates myeloid cells. J Immunol. 2001; 167(1):336-343. (Biology). View Reference
Rochman I, Watanabe N, Arima K, Liu YJ, Leonard WJ. Cutting edge: direct action of thymic stromal lymphopoietin on activated human CD4+ T cells. J Immunol. 2007; 178(11):6720-6724. (Biology). View Reference
Russell LJ, Capasso M, Vater I, et al. Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. Blood. 2009; 114(13):2688-2698. (Biology). View Reference
Tedla N, Bandeira-Melo C, Tassinari P, et al. Activation of human eosinophils through leukocyte immunoglobulin-like receptor 7. Proc Natl Acad Sci U S A. 2003; 100(3):1174-1179. (Biology). View Reference
Ziegler SF. Thymic stromal lymphopoietin and allergic disease. J Allergy Clin Immunol. 2012; 130(4):845-852. (Biology). View Reference

View All (10)

563149 Rev. 1

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