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      PE Mouse anti-Human CD238
      PE Mouse anti-Human CD238
      Flow cytometric analysis of CD238 expression on human erythrocytes.  Human erythrocytes were stained with the PE Mouse Anti-Human CD238 antibody (Cat. No. 563122; solid line histogram) or with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of erythrocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
      PE Mouse anti-Human CD238
      Flow cytometric analysis of CD238 expression on human erythrocytes.  Human erythrocytes were stained with the PE Mouse Anti-Human CD238 antibody (Cat. No. 563122; solid line histogram) or with PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of erythrocytes. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System.
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      BD Pharmingen™
      KEL; KELL; Kell blood group, metallo-endopeptidase; ECE3
      Human (QC Testing)
      Mouse IgG1, κ
      Human erythrocytes
      Flow cytometry (Routinely Tested)
      5 µl
      VII 70565
      AB_2738017
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563122 Rev. 1
      Antibody Details
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      BRIC 203

      The BRIC 203 monoclonal antibody specifically binds to the Kell blood group antigen, Kp(a-b+) but not Kp(a+b-),  also known as the Kell blood group metallo-endopeptidase, KELL, ECE3 and CD238. CD238 is an approximately 115-kDa type II transmembrane glycoprotein that is encoded by the KEL gene. CD238 functions as a zinc endopeptidase that cleaves endothelin-3 into an active form which can function as a vasoconstrictor. CD238 is expressed on erythrocytes, human embryonic stem cell-derived endoderm and on cells from various other tissues, including testis, heart, brain and skeletal muscle. Decreased expression of CD238 is associated with the McLeod phenotype as a consequence of losing XK protein expression. Kell antigen expression can play an important role during blood transfusions and also specific autoimmune diseases related to Kell antigen recognition. During development the BRIC 203 monoclonal antibody was found to detect the CD238 antigen by flow cytometric analysis of human erythrocytes. It reportedly works in immunoprecipitation and agglutination assays.

      563122 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      563122 Rev.1
      Citations & References
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      Development References (2)

      1. Parsons SF, Gardner B, Anstee DJ. Monoclonal antibodies against Kell glycoprotein: serology, immunochemistry and quantification of antigen sites. Transfus Med. 1993; 3(2):137-142. (Immunogen). View Reference
      2. Wang P, Rodriguez RT, Wang J, Ghodasara A, Kim SK. Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm. Cell Stem Cell. 2011; 8:335-346. (Biology). View Reference
      563122 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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