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PE Mouse Anti-Human CCL19
PE Mouse Anti-Human CCL19
Flow cytometric analysis of CCL19 expression in mature human monocyte-derived dendritic cells. Monocyte- derived dendritic cells were generated from peripheral blood monocytes that were cultured (5 days) in complete RPMI medium containing BD Pharmingen™ Recombinant Human GM-CSF (Cat. No.550068, 20 ng/ml) and Recombinant Human IL-4 (Cat. No. 554605, 20 ng/ml) and restimulated (2 days) with lipopolysaccharide (100 ng/ml). The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714), and then stained with either BD Pharmingen™ PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CCL19 at 0.5 ug/test (Cat. No. 566523; solid line histogram). The histogram showing expression of human CCL19 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocyte-derived dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CCL19 expression in mature human monocyte-derived dendritic cells. Monocyte- derived dendritic cells were generated from peripheral blood monocytes that were cultured (5 days) in complete RPMI medium containing BD Pharmingen™ Recombinant Human GM-CSF (Cat. No.550068, 20 ng/ml) and Recombinant Human IL-4 (Cat. No. 554605, 20 ng/ml) and restimulated (2 days) with lipopolysaccharide (100 ng/ml). The cells were washed, fixed, and permeabilized using the BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (Cat. No. 554714), and then stained with either BD Pharmingen™ PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CCL19 at 0.5 ug/test (Cat. No. 566523; solid line histogram). The histogram showing expression of human CCL19 (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact monocyte-derived dendritic cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
CK beta-11; EBI1 ligand chemokine; ELC; MIP-3-beta; MIP3B; SCYA19
Human (QC Testing)
Mouse BALB/c IgG1, κ
Human CCL19 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2739741
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
566523 Rev. 1
Antibody Details
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T50-867

The T50-867 monoclonal antibody specifically binds to the small cytokine known as Chemokine (C-C motif) ligand 19 (CCL19). It is one of the two known ligands of the chemokine receptor CCR7; CCL21 is the other.  CCL19 is expressed in lymphoid organs (including thymus, lymph nodes, appendix and spleen), on endothelial cells at the blood-brain barrier, and in organs of the respiratory and gastrointestinal tracts. The interactions of CCL19 (and CCL21) with CCR7 control various developmental stages and migratory activities of lymphoid cells involved in adaptive immune responses.

566523 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
566523 Rev.1
Citations & References
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View product citations for antibody "566523" on CiteAb

Development References (4)

  1. Comerford I, Harata-Lee Y, Bunting MD, Gregor C, Kara EE, McColl SR. A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune system. Cytokine Growth Factor Rev. 2013; 24(3):269-83. (Biology). View Reference
  2. Forster R, Davalos-Misslitz AC, Rot A. CCR7 and its ligands: balancing immunity and tolerance. Nat Rev Immunol. 2008; 8(5):362-371. (Biology). View Reference
  3. Hauser MA, Legler DF. Common and biased signaling pathways of the chemokine receptor CCR7 elicited by its ligands CCL19 and CCL21 in leukocytes. J Leukoc Biol. 2016; 99(6):869-82. (Biology). View Reference
  4. Williams JL, Holman DW, Klein RS. Chemokines in the balance: maintenance of homeostasis and protection at CNS barriers. Front Cell Neurosci. 2014; 8:154. (Biology). View Reference
View All (4) View Less
566523 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

 

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.