The CXCR3-173 monoclonal antibody specifically binds to mouse CD183, also known as CXCR3. CD183 is a seven transmembrane spanning, G protein-coupled chemokine receptor for CXC chemokines including CXCL9 (Mig), CXCL10 (IP-10) and CXCL11 (I-TAC). These chemokines are induced by inflammatory cytokines including IFN-γ, IFN-α/β, and TNF. CXCR3 is primarily expressed on activated/memory CD4+ and CD8+ T lymphocytes, Foxp+ regulatory T cells, natural killer T (NKT) cells and mature NK cells. Binding of chemokines to CXCR3 induces integrin activation, cytoskeletal changes, and chemotactic migration of activated lymphocytes. CD183 has been reported to play important roles in T cell recruitment and immune responses in a number of inflammatory and autoimmune diseases. The CXCR3-173 antibody reportedly inhibited in vitro chemotactic responses to CXCL10 or CXCL11 significantly but not to CXCL9. When administered systemically to mouse hosts, the CXCR3-173 antibody reportedly prolonged cardiac and pancreatic islet cell allograft survival. In the presence of CXCR3 ligands, especially, CXCL10 and CXCL11, staining with the antibody can be significantly blocked.
The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm. BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor® 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.