Skip to main content Skip to navigation
Hamburger Menu
Search icon
Order lookup icon Order lookup icon
Order Lookup
Country Selector Country Selector
Canada (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      BV421 Rat Anti-Mouse CD107b
      BV421 Rat Anti-Mouse CD107b
      Flow cytometric analysis of CD107b expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, λ Isotype Control (Cat. No. 562965; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107b antibody (Cat. No. 564249; solid line histogram). The fluorescence histogram showing CD107b expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      BV421 Rat Anti-Mouse CD107b
      Flow cytometric analysis of CD107b expression in mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either BD Horizon™ BV421 Rat IgG2a, λ Isotype Control (Cat. No. 562965; dashed line histogram) or BD Horizon BV421 Rat Anti-Mouse CD107b antibody (Cat. No. 564249; solid line histogram). The fluorescence histogram showing CD107b expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of intact leucocytes. Flow cytometric analysis was performed using a BD™ LSR II Flow Cytometer System.
      Product Details
      Down Arrow Up Arrow


      BD Horizon™
      Lamp2; LAMP-2; LAMP II; Lysosomal-associated membrane protein 2; LGP-B
      Mouse (QC Testing)
      Rat SD, also known as Sprague-Dawley (outbred) IgG2a, λ
      Glycoproteins purified from BALB/c mouse embryo 3T3 cell line
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      AB_2738702
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      Show More blue down arrow Show Less blue up arrow
      564249 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      ABL-93

      The ABL-93 monoclonal antibody specifically recognizes CD107b which is also known as Lysosome-associated membrane protein 2 (LAMP2/LAMP-2), Lysosomal membrane glycoprotein type B (LGP-B), and Mac-3. CD107b is a type I lysosomal transmembrane glycoprotein that serves as a useful marker to distinguish lysosomes from other cellular organelles. Mouse CD107b consists of a ~40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoproteins of 110-120 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice.

      The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

      564249 Rev. 1
      Format Details
      Down Arrow Up Arrow
      BV421
      The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      BV421
      Violet 405 nm
      407 nm
      423 nm
      564249 Rev.1
      Citations & References
      Down Arrow Up Arrow

      Development References (3)

      1. Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990; 265(9):5008-5013. (Biology). View Reference
      2. Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986; 51:97-112. (Clone-specific: Fluorescence microscopy, Immunofluorescence). View Reference
      3. Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985; 101(1):85-95. (Immunogen: Fluorescence microscopy, Immunofluorescence, Immunoprecipitation, Radioimmunoassay). View Reference
      564249 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

      Website Feedback