Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The HI9A monoclonal antibody specifically recognizes CD9 which is also known as Motility related protein-1 (MRP-1), Tetraspanin-29 (Tspan-29, TSPAN29), and Leukocyte antigen MIC3 (MIC3). CD9 is a 24 kDa type III transmembrane protein that is encoded by CD9 which belongs to the transmembrane 4 superfamily. CD9 is a tetraspanin composed of four transmembrane and two extracellular domains with short intracellular N- and C-termini. CD9 is expressed by platelets and variably expressed by pre-B cells, activated B cells and plasma cells, activated T cells, monocytes, macrophages, resting and activated neutrophils, eosinophils, basophils, mast cells, endothelial and epithelial cells, neurons and glial cells as well as some malignant cells and cell lines. CD9 has multiple functions and associates with integrins, MHC class II molecules in addition to other tetraspanins and molecules to mediate cellular signal transduction, adhesion, and migration responses. CD9 associates with the αIIb/β3 integrin (CD41/CD61) on platelets and regulates their activation and aggregation. CD9 can also co-stimulate T cell responses and mediate egg:sperm adhesion and fusion in the fertilization process. CD9 serves as a marker that is expressed on the surface of exosomes.
Development References (10)
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Akuthota P, Melo RC, Spencer LA, Weller PF. MHC Class II and CD9 in human eosinophils localize to detergent-resistant membrane microdomains.. Am J Respir Cell Mol Biol. 2012; 46(2):188-95. (Biology). View Reference
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Brosseau C, Colas L, Magnan A, Brouard S. CD9 Tetraspanin: A New Pathway for the Regulation of Inflammation?. Front Immunol. 2018; 9:2316. (Biology). View Reference
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Cramer EM, Berger G, Berndt MC. Platelet alpha-granule and plasma membrane share two new components: CD9 and PECAM-1. Blood. 1994; 84(6):1722-1730. (Biology). View Reference
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Hawke LG, Whitford MKM, Ormiston ML. The Production of Pro-angiogenic VEGF-A Isoforms by Hypoxic Human NK Cells Is Independent of Their TGF-β-Mediated Conversion to an ILC1-Like Phenotype.. Front Immunol. 2020; 11:1903. (Clone-specific: Flow cytometry). View Reference
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Hill DA, Lim HW, Kim YH, et al. Distinct macrophage populations direct inflammatory versus physiological changes in adipose tissue.. Proc Natl Acad Sci U S A. 2018; 115(22):E5096-E5105. (Clone-specific: Flow cytometry). View Reference
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Masellis-Smith A, Shaw AR. CD9-regulated adhesion. Anti-CD9 monoclonal antibody induce pre-B cell adhesion to bone marrow fibroblasts through de novo recognition of fibronectin. J Immunol. 1994; 152(6):2768-2777. (Biology). View Reference
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Silverstein RL. Platelet antigens: Section report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1197-1204.
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Tai XG, Toyooka K, Yashiro Y, et al. CD9-mediated costimulation of TCR-triggered naive T cells leads to activation followed by apoptosis. J Immunol. 1997; 159:3799-3807. (Biology).
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Yoon SO, Zhang X, Lee IY, Spencer N, Vo P, Choi YS. CD9 is a novel marker for plasma cell precursors in human germinal centers.. Biochem Biophys Res Commun. 2013; 431(1):41-6. (Biology). View Reference
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Zhang C, Fan Y, Che X, et al. Anti-PD-1 Therapy Response Predicted by the Combination of Exosomal PD-L1 and CD28.. Front Oncol. 2020; 10:760. (Clone-specific: Flow cytometry). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.