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      Mouse Hematopoetic Stem Cell SLAM Code Panel

      BD Stemflow™ Mouse Hematopoetic Stem Cell SLAM Code Panel

      (RUO)
      Mouse Hematopoetic Stem Cell SLAM Code Panel
      Multicolor flow cytometric analysis of bone marrow hematopoietic stem cells. BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE Hamster Anti-Mouse CD48 (Cat. No. 557485), and Alexa Fluor® 647 Rat Anti-Mouse CD150 (Cat. No. 562647) antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P4 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated), Lineage-negative (Left Plot, P2 Gate) and CD48-CD150+ (Middle Plot, P3 Gate) cells. Lineage-CD41-CD48-CD150+ bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells.
      Mouse Hematopoetic Stem Cell SLAM Code Panel
      Multicolor flow cytometric analysis of bone marrow hematopoietic stem cells. BALB/c mouse bone-marrow cells were labeled with the BD IMag™ Mouse Hematopoietic Progenitor Enrichment Set (Cat. No. 558451) and separated on the BD IMag™ Cell Separation Magnet (Cat. No. 552311) according to the set protocol. The non-depleted bone marrow cells were subsequently stained with BD Horizon™ V450 Mouse Lineage Antibody Cocktail (Cat. No. 561301) and FITC Rat Anti-Mouse CD41 (Cat. No. 553848/561849), PE Hamster Anti-Mouse CD48 (Cat. No. 557485), and Alexa Fluor® 647 Rat Anti-Mouse CD150 (Cat. No. 562647) antibodies. A fluorescence histogram (Right Plot) shows little or no CD41 expression (P4 Gate) by bone marrow cells that were progressively gated as viable (light scatter gated), Lineage-negative (Left Plot, P2 Gate) and CD48-CD150+ (Middle Plot, P3 Gate) cells. Lineage-CD41-CD48-CD150+ bone marrow cells have been reported to be highly enriched for adult mouse hematopoietic stem cells.
      Product Details
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      BD Stemflow™
      Flow cytometry (Routinely Tested)
      RUO
      AB_2869420


      Description

      The Q38-480 monoclonal antibody specifically binds to mouse CD150, also known as SLAM (signaling lymphocyte activation molecule). CD150 is a type 1 transmembrane glycoprotein that is a member of the CD2 subfamily of the Ig superfamily. It is encoded by the Slamf1 (signaling lymphocytic activation molecule family member 1) gene. CD150 is differentially expressed on subsets of thymocytes, T and B lymphocytes, dendritic cells, macrophages, and endothelial cells. SLAM plays multiple roles in innate and adaptive immunity serving as an adhesion molecule and/or coreceptor. CD150-mediated costimulation of TCR-activated T cells reportedly results in the increased production of IFN-γ by Th1 cells and is required for IL-4 production by T follicular helper cells. CD150 also plays important roles in hematopoietic cell developmental pathways. CD150 is differentially expressed by self-renewing  adult hematopoietic stem cells (HSC) that are CD150+ whereas non-multipotent hematopoietic progenitor cells are CD150-. Utilizing additional cell surface markers, lineage-negative CD150+CD48-CD41- cell fractions are reported to be highly enriched for adult HSC.

      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
      5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
      9. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
      10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      562461 Rev. 5
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      Alexa Fluor® 647 Rat anti-Mouse CD150 50 µg (1 ea) 562647 N/A
      PE Hamster Anti-Mouse CD48 50 µg (1 ea) 562398 N/A
      FITC Rat Anti-Mouse CD41 50 µg (1 ea) 561849 N/A
      562461 Rev. 5
      Citations & References
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      Development References (5)

      1. Castro AG, Hauser TM, Cocks BG, et al. Molecular and functional characterization of mouse signaling lymphocytic activation molecule (SLAM): differential expression and responsiveness in Th1 and Th2 cells.. J Immunol. 1999; 163(11):5860-70. (Immunogen: (Co)-stimulation, Flow cytometry, Immunoprecipitation, Stimulation). View Reference
      2. Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C, Morrison SJ. SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell. 2005; 121(7):1109-1121. (Biology). View Reference
      3. Weksberg DC, Chambers SM, Boles NC, Goodell MA. CD150- side population cells represent a functionally distinct population of long-term hematopoietic stem cells. Blood. 2008; 111(4):2444-2451. (Biology). View Reference
      4. Wilson A, Laurenti E, Oser G, et al. Hematopoietic stem cells reversibly switch from dormancy to self-renewal during homeostasis and repair.. Cell. 2008; 135(6):1118-1129. (Biology). View Reference
      5. Yilmaz OH, Kiel MJ, Morrison SJ. SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood. 2006; 107(3):924-930. (Biology). View Reference
      562461 Rev. 5

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.