BD FACSLyric™ Clinical System

The power to transform

Discover the difference of the BD FACSLyric™ flow cytometry

  • Witness clinical performance results you have never seen before, through high sensitivity and improved resolution
  • Streamline your lab workflow through flexibility and automation, enabling efficiency and productivity
  • Achieve automated standardization through highly reproducible results and enable collaboration through assay portability

See how the BD FACSLyric system can transform your lab


Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use. CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC
Overview Performance Flexibility & Automation Standardization Software

The next generation of flow cytometry

The BD FACSLyric flow cytometry solution combines simplicity, speed and automation to ease workflow and improve productivity. This next-generation flow cytometer enables standardization and collaboration through consistent results and unique assay portability capabilities.

Built on a foundation of excellence, experience and expertise, the BD FACSLyric is a new diagnostic standard for clinical cell analysis, transforming the way your lab does flow cytometry. As with all BD instruments, the BD FACSLyric is backed by 40 years of BD expert training, service and support—so there’s no limit to your potential.

Just the FACS

  • 4-, 6-, 8-, 10- and 12-color configurations. Onsite upgradeable to adapt to your lab's changing needs
  • Up to 3 lasers—blue, red and violet—12 fluorescence channels and 14 parameters
  • 35,000 events per second maximum acquisition rate; no limit on number of events acquired
  • Automated single-tube QC with BD™ CS&T beads1
  • Fluorescence compensation required only every 60 days, improving efficiency and productivity
  • 21 different loading options: plates or tubes; built-in flexibility with BD FACS™ Universal Loader1

External Components

Hover over the orange prompts to view key features. Additional information is available in our technical specifications.


Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use. CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC
1 For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.  Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

A new diagnostic standard delivering outstanding performance

The BD FACSLyric is a high-performance, highly sensitive flow cytometer that demonstrates exceptional resolution and improved separation to make dim and rare populations easier to resolve.


Runs at rates up to 35,000 events per second, sample carryover ≤ 0.05%.

  • Acquires a large number of events rapidly; useful for rare populations.
  • There is no limit on events acquired.

Outstanding resolution at all flow rates.

  • Enables faster detection without compromising quality. (Figure 1) 
    FACSLyric - Features - Flow Rate 
    Figure 1. Flow rate

Improvement in stain index of 8-190% across all parameters.

  • Better separation enables faster analysis and easier gating. (Figure 2) 

    FACSLyric - Features - Stain Index


    Figure 2. Peak resolution of BD FACSLyric vs the BD FACSCanto™ II system



  • Higher sensitivity makes dim and rare populations easier to resolve. (Figure 3)
    FACSLyric - Features - Sensitivity

    Figure 3. Improved resolution of dim population


Class 1 Laser Product.
The BD FACSLyric™ and BD FACSCanto™ II flow cytometer are for In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC

Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

Improving efficiency and productivity through flexibility and automation

ntelligent system design simplifies your laboratory workflow through built-in flexibility and automation thus enabling your technicians to be more productive.


FACSLyric RUO - Features - Every Technician - Loader

A lab-friendly system, with a small footprint, quiet operation and the BD FACS™ Universal Loader

  • Automated loader can accommodate 30 or 40 tubes 1
  • 21 different loading options
    • 3 types of tube racks
    • 16 types of 96-well plates
    • 2 types of 384-well plates
  • Automated vortexing of tubes and plates for mixing and resuspending
  • Automated sample tracking tubes and plates (barcode enabled)
  • Compact instrument size 63.2 x 57.9 x 57.9 cm (24.9 x 22.8 x 22.8 in.) for smaller lab spaces
  • Noise levels of ≤55 dBA for a quieter lab environment

FACSLyric - Features - Flexibility - FC Bead Tubes

Patented BD™ bead technology enables data reproducibility

  • Daily setup and performance checks using BD CS&T beads 1 automatically adjust more than 70 instrument parameters ensuring stable instrument performance with CV of <0.4%
  • PMT voltages are automatically updated to maintain target MFI values as a part of QC
  • Spillover values (SOVs) are automatically updated as part of daily QC
  • Compensation only needs to be performed every 2 months.

Spend zero minutes on startup and shutdown

  • Pre-programmable startup means instruments are ready to run the minute users walk in.
  • Auto shutdown and power off also contributes to maximizing instrument productivity.

FACSLyric - Features - Automation


Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC

Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

1 For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.  Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

Elevating standardization and collaboration to a new level

The BD FACSLyric is designed to enable assay reproducibility across instruments and time.


Universal Setup ensures reproducible and accurate results day to day with stable instrument performance of <0.4%. (Figure 4)

  • Universal Setup, a series of built-in daily performance checks
  • Optimal instrument performance is achieved through one-tube daily CS&T 1 as part of universal setup

FACSLyric - Standardization - Fig 4

Figure 4: Within-instrument reproducibility of CS&T 1 target MFI's.  
For each fluorescence channel, the median of BD™ CS&T 1 bright beads MFI generated from the BD FACSLyric assay setup reports was compared with assay reference target values. The % difference between target and measured was calculated and is shown.

The BD FACSLyric was designed with collaboration in mind.

The unique assay and portability feature enables the sharing and exchange of data, ideas and user-defined protocols within and between institutions.

  • User-defined assays are saved and electronically transferred via USB or email to another FACSLyric system
  • The time required to set up additional instruments is reduced
  • Assay portability simplifies and standardizes instrument setup within your lab and between labs, making collaboration effective and efficient

FACSLyric - Standardization - Fig 5

Figure 5: User-defined assay across three instruments shows the reproducible and accurate performance of the BD FACSLyric Universal Setup.

Assay portability and sharing in 4 easy steps

FACSLyric -Standardization - Assay Portability 

Strengthening partnerships and expanding global collaborations through assay portability and sharing.

FACSLyric - Standardization - Collaboration 

Highly reproducible results between instruments drive standardization (see Table 1 and Figure 6)

BD FACSLyric Variance (CVs)
15 BD FACSLyric systems: Lyse/Wash settings and BD FC beads
Blue laser %CV Red laser %CV Violet laser %CV
FITC 4.2 APC 5.3 V450 11.1
PE 4.4 APC-Cy7 4.1 V500 11
PE-Cy7 4.8 APC-H7 3.9 BV605 13.6
PerCP 10.2 APC-R700 4.2 BV711 7.5
PerCp-Cy5.5 7.9     BV786 15.3

Table 1. Between-instrument reproducibility of target MFI values on the BD FACSLyric 
Lyse/wash assay settings were imported across 15 instruments to show effects of standardization on beads. the CVs of the fluorescence intensity across all channels varies by less than 15.3%. (Figure 5) Daily QC with one lot of BD CS&T 1 beads was run on fifteen BD FACSLyric cytometers. For each instrument, the PMTV gains were automatically adjusted to meet the target values. BD FC beads 1 acquired on each BD FACSLyric instrument. The MFI of positive populations was measured for all parameters across all instruments. The %CV is shown.

The data for this internal study was acquired using BD FC Beads 1 across 15 instruments. Greater between-instrument variability could be observed when running biological samples, when using non-BD reagents or when comparing fewer instruments.


Copy of FACSLyric - Standardization - Fig 6

Figure 6: Corresponding overlays of 15 BD FACSLyric systems show between-instrument variability of less than 8% for the red and blue channels


Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC

Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

1 For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.  Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

Data management software to complement your workflow

The BD FACSLyric powered by the BD FACSuite software streamlines instrument setup and takes analysis to a new level, providing the flexibility you need for panel design and data visualization. FACSLink and Assurity Linc software integration streamline your workflow, helping to improve productivity.


BD FACSuite acquisition and analysis software is robust and reliable, helps to achieve 21 CFR Part 11 compliance.

  • Password protection, audit trail, electronic signatures and IQ/OQ procedures assist in supporting 21 CFR Part 11 compliance and electronic record integrity
  • BD FACSuite software consists of two modules. Both modules have a common user interface and identical menu navigation.
  • Reports are customizable with tables, headers and footers.
  • Built-in flexibility for creating customized calculations and expressions.
  • With Microsoft®Windows® 10 improved security, BD FACSuite software is available for office analysis.

For more information on FACSuite Clinical software1 click here.

For more information on FACSuite software click here.

Integrated solutions to optimize lab productivity and secure data from request to reporting

  • Seamless laboratory information system (LIS) integration enabled by the FACSLink™ software interface solution reduces transcription errors and improves laboratory efficiency.
  • Bidirectional transfer of information between your LIS and the BD FACSLyric using the BD FACSLink interface solution reduces transcription errors.
  • Remote diagnostics and support with BD Assurity Linc™ enable identification of maintenance needs and off-site technical support.

FACSLyric - Features - Software Schematic - Lrg 

For more information on FACSLink software interface solution click here.


Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use. CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC
1 For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.  Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

A Powerful Solution for CD4 monitoring.

With the BD FACSLyric™ system, clinical laboratories can obtain repeatable and reliable results for CD4 monitoring and immune status analysis.


BD FACSLyric™ provides a solution for CD4 assays, delivering improved clinical performance and accuracy in diagnosis with increased process efficiency and standardization.

  • BD Tritest™ and BD Multitest™ reagents1, formulated to be used with BD Trucount™ tubes1, provide absolute counting capability
  • BD FACSuite™ clinical software1, with predefined templates for 3, 4, and 6-color TBNK analysis, provides reproducible and consistent results
  • Each reagent includes a cocktail of multiple fluorescently-labeled monoclonal antibodies, premixed at the appropriate titer to ensure quality staining
FACSLyric - Applications - Sample Data

Streamlined methodology for ease of use

  1. Add 20 µL of BD Tritest1 or BD Multitest™ reagent1 to a BD Trucount™ tube1
  2. Add 50 µL of well-mixed whole blood and vortex gently to mix
  3. Incubate in the dark for 15 minutes
  4. Add 450 µL of BD FACS™ Lysing solution1 and vortex gently to mix
  5. Incubate in the dark for 15 minutes
  6. Analyze sample on BD FACSLyric™ system

Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use. CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC
1 For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.  Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

BD FACSLyric™ System Testimonials

See what customers are saying about the BD FACSLyric™, our next generation flow cytometry solution that enables standardization and collaboration through consistent results and unique assay portability capabilities.

FACSLyric - Testimonials - Dr. Hoffman “The BD FACSLyric is a system for everyday work that every technician can use.”

—Dr. Dennis Hoffman, Labor Wisplinghoff, Cologne, Germany
FACSLyric - Testimonials - Dr. Bray “The flow crossmatch market may be one of the smallest, but standardization has the potential to have one of the greatest clinical impacts. It’s the little things that make a big difference in healthcare.”

—Dr. Robert Bray, Emory University, Atlanta, GA
FACSLyric - Testimonials - Dr. Wang “BD is positioned with BD™ CS&T and BD™ FC bead technology to enable instrument standardization simply from day to day, instrument to instrument, and lab to lab. Reference control based instrument standardization is the most important “next step” for clinical flow cytometry."

—Dr. Lili Wang, NIST, Gaithersburg, MD
FACSLyric - Testimonials - Dr. Kromminga “The consistency and the regulatory compliance of FACSLyric’s output is what really drew us to the platform. It is currently unmatched by other flow cytometers in its ability to generate reliable, repeatable results across tests and instruments – even for the most complex assays we deal with day to day. We’ve found it particularly beneficial for multi-parameter testing for CAR T cells and immunophenotyping in immune-oncology for non-clinical and clinical analysis.”

—Dr. Arno Kromminga, Chief Scientific Officer, BioAgilytix Europe
FACSLyric - Testimonials - Dr. Moser “The assay portability of BD FACSLyric is the key to efficient method transfer and high data reproducibility. It represents a clear advantage in a regulated environment, where conditions must be met to produce valid results of a guaranteed level of quality. Functional immune cell-based assays and cytokine quantification in pre-clinical and clinical drug development is a particularly important area where BD FACSLyric offers clear technical advantages.”

—Dr. René Moser, Chief Executive Officer & Chief Scientific Officer, IBR Inc., Switzerland
FACSLyric - Testimonials - Christian Smith “[The FACSLyrics] are very specific and precise with their testing which is excellent, it means we are getting better results overall. ”

—Christian Smith, Senior Scientist; Sullivan Nicolaides Pathology, Australia
Testimonial: Christian Smith
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1For In Vitro Diagnostic Use CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC.   Unless otherwise specified, all products are for research use only. Not for use in diagnostic and therapeutic procedure

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Class 1 Laser Product.
The BD FACSLyric™ flow cytometer is for In Vitro Diagnostic Use. CE marked in compliance with the European In Vitro Medical Device Directive 98/79/EC