The Custom Technology Team works with clients to make both qualitative and quantitative multicolor antibody cocktails for flow cytometry–based immunophenotyping.
These products include conjugated antibodies, compensation controls, buffers, and protocol guidelines. Optimized multicolor flow cytometry cocktails can streamline sample preparation, acquisition, and analysis, and improve standardization between experiments.
Intracellular proteomics (BD Phosflow)
CTT offers assay optimization, sample processing, and data delivery for BD™ Phosflow analysis. An intracellular phosphoprofiling tool, BD Phosflow technology allows scientists to examine cellular subpopulations in complex samples such as primary cells, whole blood, or peripheral blood mononuclear cells (PBMCs). Unlike traditional methods such as Western blotting, BD Phosflow enables researchers to analyze phosphoprotein signaling in single cells through the use of multiple cell surface markers. Using BD Phosflow alone, researchers can uncover rare cell subtypes with different signaling mechanisms.
Cell surface marker profiling
For researchers using stem cells in drug discovery or cell therapy, CTT offers cell surface marker profiling to identify cell state variations indicative of donor-to-donor variations, cell type specific markers, or treatment-specific changes. The team leverages the novel proteomics tool BD FACS™ CAP (Combinatorial Antibody Profiling), a flow cytometry–based method that uses a broad screening panel of more than 200 high-quality fluorescently-labeled BD antibodies.