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Purified Mouse Anti-ZRP-1
Purified Mouse Anti-ZRP-1
Western blot analysis of ZRP-1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- ZRP-1 antibody.
Western blot analysis of ZRP-1 on a BC3H1 cell lysate (Mouse brain smooth muscle-like cells; ATCC CRL-1443).  Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- ZRP-1 antibody.
Product Details
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BD Transduction Laboratories™
Zyxin Related Protein-1; TRIP6; Thyroid Receptor Interacting Protein-6
Mouse (QC Testing), Human, Rat (Tested in Development)
Mouse IgG1
Human ZRP-1 aa. 103-209
Western blot (Routinely Tested), Immunofluorescence (Not Recommended)
51 kDa
250 µg/ml
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
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Antibody Details
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Zyxin is a zinc-binding metalloprotein found in focal adhesion plaques. It contains a proline-rich N-terminal domain and a C-terminal domain containing three LIM domains.  LIM domains are characterized as zinc-binding motifs involved in gene expression and protein-protein interactions.  Zyxin related protein 1 (ZRP-1) was identified through its interaction with the second PDZ domain of PTP1E.  ZRP-1 has also been identified as Thyroid Receptor Interacting Protein-6 (TRIP6) during screening for binding partners of the thyroid hormone receptor.  ZRP-1 contains an N-terminal proline-rich region (PRR), and three double zinc finger LIM domains in the C-terminal region.  ZRP-1 mRNA has been reported to be widely expressed with strong expression in heart, placenta, lung, liver, kidney, and pancreas.  ZRP-1 may interact with focal adhesions similar to other LIM domain proteins in the zyxin-related subfamily.  However, ZRP-1 also contains nuclear export signal, and treatment of cells with leptomycin B leads to movement of ZRP-1 from the cytoplasm to the nucleus.  Thus, ZRP-1 may be important for relaying signals from focal adhesions to the nucleus.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.

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Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
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Citations & References
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Development References (2)

  1. Murthy KK, Clark K, Fortin Y, Shen SH, Banville D. ZRP-1, a zyxin-related protein, interacts with the second PDZ domain of the cytosolic protein tyrosine phosphatase hPTP1E. J Biol Chem. 1999; 274(29):20679-20687. (Biology). View Reference
  2. Wang Y, Gilmore TD. LIM domain protein Trip6 has a conserved nuclear export signal, nuclear targeting sequences, and multiple transactivation domains. Biochim Biophys Acta. 2001; 1538(2-3):260-272. (Biology). View Reference
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Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.