BD Transduction Laboratories™ Purified Mouse Anti-ZRP-1

Zyxin is a zinc-binding metalloprotein found in focal adhesion plaques. It contains a proline-rich N-terminal domain and a C-terminal domain containing three LIM domains.  LIM domains are characterized as zinc-binding motifs involved in gene expression and protein-protein interactions.  Zyxin related protein 1 (ZRP-1) was identified through its interaction with the second PDZ domain of PTP1E.  ZRP-1 has also been identified as Thyroid Receptor Interacting Protein-6 (TRIP6) during screening for binding partners of the thyroid hormone receptor.  ZRP-1 contains an N-terminal proline-rich region (PRR), and three double zinc finger LIM domains in the C-terminal region.  ZRP-1 mRNA has been reported to be widely expressed with strong expression in heart, placenta, lung, liver, kidney, and pancreas.  ZRP-1 may interact with focal adhesions similar to other LIM domain proteins in the zyxin-related subfamily.  However, ZRP-1 also contains nuclear export signal, and treatment of cells with leptomycin B leads to movement of ZRP-1 from the cytoplasm to the nucleus.  Thus, ZRP-1 may be important for relaying signals from focal adhesions to the nucleus.

This antibody is routinely tested by western blot analysis.  Other applications were tested in BD Biosciences Pharmingen during antibody development only or reported in the literature.