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Targeted mRNA and AbSeq Amplification Kit - 4 pack

Targeted mRNA and AbSeq Amplification Kit - 4 pack

(RUO)
Product Details
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BD Rhapsody™
Single cell multiomics experiments on the BD Rhapsody™ platform
RUO


Description

The purpose of this kit is to provide the necessary reagents for the preparation of single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system.This catalog number covers all the 4 kits needed for your single cell Targeted mRNA experiments and includes BD Rhapsody™ Cartridge Reagent Kit (cat no. 633731); BD Rhapsody™ Cartridge Kit (cat no. 633733); BD Rhapsody™ cDNA Kit (cat no. 633773); BD  Rhapsody™ Targeted mRNA and AbSeq Amplification Kit  (cat no. 633774)


The purpose of this kit is to provide the necessary reagents for the preparation of single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system.This catalog number covers all the 4 kits needed for your single cell Targeted mRNA experiments and includes BD Rhapsody™ Cartridge Reagent Kit (cat no. 633731); BD Rhapsody™ Cartridge Kit (cat no. 633733); BD Rhapsody™ cDNA Kit (cat no. 633773); BD  Rhapsody™ Targeted mRNA and AbSeq Amplification Kit  (cat no. 633774)

Preparation And Storage

Store the BD Rhapsody™ Targeted mRNA and AbSeq Amplification Kit and BD Rhapsody™ cDNA Kit at –25°C to –15°C. Store the BD Rhapsody™ Cartridge Reagent Kit 2°C to 8°C and the BD Rhapsody™ Cartridge Kit at room temperature (15°C to 25°C ).

633771 Rev. 1
Components
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Description Quantity/Size Part Number
Cartridge Reagent Kit 1 Each (1 ea) 633731
Cartridge Kit 1 Each (1 ea) 633733
cDNA Kit 1 Each (1 ea) 633773
Targeted mRNA and AbSeq Amplification Kit 1 Each (1 ea) 633774
633771 Rev. 1
Citations & References
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Development References (14)

  1. Alivernini S, MacDonald L, Elmesmari A, et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020; 26(8):1295-1306. (Biology). View Reference
  2. Arroyo Hornero R, Georgiadis C, Hua P, et al. CD70 expression determines the therapeutic efficacy of expanded human regulatory T cells. Commun Biol. 2020; 3(1):375. (Biology). View Reference
  3. Erickson JR, Mair F, Bugos G, et al. AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level. STAR Protoc. 2020; 1(2):100092. (Biology). View Reference
  4. Gertz ML, Chin CR, Tomoiaga D, et al. Multi-omic, Single-Cell, and Biochemical Profiles of Astronauts Guide Pharmacological Strategies for Returning to Gravity. Cell Rep. 2020; 33(10):108429. (Biology). View Reference
  5. Jensen-Cody SO, Flippo KH, Claflin KE, et al. FGF21 Signals to Glutamatergic Neurons in the Ventromedial Hypothalamus to Suppress Carbohydrate Intake. Cell Metab. 2020; 32(2):273-286. (Biology). View Reference
  6. Li S, Simoni Y, Becht E, et al. Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer. Cell Rep Med. 2020; 1(3):100039. (Biology). View Reference
  7. Mair F, Erickson JR, Voillet V, et al. A Targeted Multi-omic Analysis Approach Measures Protein Expression and Low-Abundance Transcripts on the Single-Cell Level. Cell Rep. 2020; 31(1):107499. (Biology). View Reference
  8. Malkani S, Chin CR, Cekanaviciute E, et al. Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development. Cell Rep. 2020; 33(10):108448. (Biology). View Reference
  9. Mohebiany AN, Ramphal NS, Karram K, et al. Microglial A20 Protects the Brain from CD8 T-Cell-Mediated Immunopathology. Cell Rep. 2020; 30(5):1585-1597. (Biology). View Reference
  10. Sainson RCA, Thotakura AK, Kosmac M, et al. An Antibody Targeting ICOS Increases Intratumoral Cytotoxic to Regulatory T-cell Ratio and Induces Tumor Regression. Cancer Immunol Res. 2020; 8(12):1568-1582. (Biology). View Reference
  11. Tang X, Zhang S, Peng Q, et al. Sustained IFN-I stimulation impairs MAIT cell responses to bacteria by inducing IL-10 during chronic HIV-1 infection. Sci Adv. 2020; 6(8):eaaz0374. (Biology). View Reference
  12. Trzupek D, Dunstan M, Cutler AJ, et al. Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis. Genome Med. 2020; 12(1):55. (Biology). View Reference
  13. Wagar LE, Salahudeen A, Constantz CM, et al. Modeling human adaptive immune responses with tonsil organoids. Nat Med.. 2021; 27(1):125-135. (Biology). View Reference
  14. Yang Y, Liu F, Liu W, et al. Analysis of single-cell RNAseq identifies transitional states of T cells associated with hepatocellular carcinoma. Clin Transl Med. 2020; 10(3):e133. (Biology). View Reference
View All (14) View Less
633771 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.