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Purified Mouse Anti-Human CD11c for IHC
Purified Mouse Anti-Human CD11c for IHC

Immunohistochemical staining of CD11c positive cells. Frozen sections of normal human spleen was stained with Purified Mouse Anti-Human CD11c antibody. Cells positive for CD11c can be identified by the intense brown labeling if their cell membranes. Amplification 20X.

Immunohistochemical staining of CD11c positive cells. Frozen sections of normal human spleen was stained with Purified Mouse Anti-Human CD11c antibody. Cells positive for CD11c can be identified by the intense brown labeling if their cell membranes. Amplification 20X.

Product Details
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BD Pharmingen™
ITGAX; AlphaX integrin chain; Axb2; Integrin alpha-X; CR4; Leu M5; SLEB6
Human (QC Testing)
Mouse BALB/c IgG1, κ
Spleen Cells from Human with Hairy Cell Leukemia
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development), Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
31.25 µg/ml
IV N012
3687
AB_393646
Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Recommended Assay Procedures

Immunohistochemistry: The B-ly6 antibody is recommended to test for immunohistochemical staining of acetone-fixed frozen sections. Tissue tested was human spleen and tonsil. The antibody stains granulocytes, monocytes, NK cells and a subset of B- and T-cells. For optimal indirect immunohistochemical staining, the B-ly6 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) (Cat. No. 550337) as the secondary antibody and Streptavidin-HRP (Cat. No. 550946) together with the DAB detection system (Cat. No. 550880). The clone B-ly6 is not recommended for formalin-fixed paraffin embedded sections. For more protocol information please visit http://www.bdbiosciences.com/resources/cellbiology/index.jsp

Other applications: The B-ly6 antibody is routinely tested by immunofluorescent staining for flow cytometric analysis and is available in different fluorochrome conjugated formats.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. This antibody has been developed for the immunohistochemistry application. However, a routine immunohistochemistry test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
555390 Rev. 9
Antibody Details
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B-ly6

The B-ly6 monoclonal antibody specifically binds to the 150 kDa adhesion glycoprotein CD11c (p150, integrin α chain). CD11c is expressed on dendritic cells, monocytes, macrophages, granulocytes, NK cells and subsets of B and T cells. It associates with CD18 to form the CD11c/CD18 complex that binds fibrinogen and has been reported to be a receptor for iC3b and ICAM-1. Reports indicate that CD11c/CD18 plays a role as an adhesion molecule that mediates cellular binding to ligands expressed on stimulated epithelium and endothelium.

555390 Rev. 9
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555390 Rev.9
Citations & References
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Development References (4)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
  3. Stacker SA, Springer TA. Leukocyte integrin P150,95 (CD11c/CD18) functions as an adhesion molecule binding to a counter-receptor on stimulated endothelium. J Immunol. 1991; 146(2):648-655. (Biology). View Reference
  4. Visser L, Shaw A, Slupsky J, Vos H, Poppema S. Monoclonal antibodies reactive with hairy cell leukemia. Blood. 1989; 74(1):320-325. (Immunogen: Immunocytochemistry (cytospins), Immunofluorescence, Immunohistochemistry, Immunoprecipitation). View Reference
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555390 Rev. 9

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.