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Two-color analysis of the expression of Vβ 7 TCR on peripheral T lymphocytes. BALB/c lymph node cells were simultaneously stained with PE-conjugated TR310, FITC-conjugated RM4-5 (anti-CD4, Cat. No. 553046/553047), and FITC-conjugated 53-6.7 (anti-CD8a, Cat. No. 553030/553031) monoclonal antibodies. Flow cytometry was performed on a FACScan™ (BDIS, San Jose, CA).
BD Pharmingen™ PE Rat Anti-Mouse Vβ 7 T-Cell Receptor
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
For flow cytometry of cell suspensions from peripheral lymphoid tissues, it is recommended that multicolor staining be performed to distinguish T lymphocytes from non-T cells.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
The TR310 monoclonal antibody specifically recognizes the Vβ 7 T-Cell Receptor (TCR) of strains having the a (e.g., C57BR, C57L, SJL), b (e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ 7 is among the predominant TCRs expressed by NK-T cells of C57BL/6 mice. Vβ 7 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigens encoded by Mtv-7 (Mls-1[a], Mls[a]) endogenous proviruses (e.g., AKR, CBA/J, C58, DBA/2, NZB). Activation or elimination of Vβ 7 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of Vβ 7 TCR-bearing T cells.
Development References (4)
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Bendelac A. Mouse NK1+ T cells. Curr Opin Immunol. 1995; 7(3):367-374. (Biology). View Reference
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Hodes RJ, Abe R. Mouse endogenous superantigens: Ms and Mls-like determinants encoded by mouse retroviruses.. Curr Protoc Immunol. 2001; Appendix 1:Appendix 1F. (Biology). View Reference
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Okada CY, Holzmann B, Guidos C, Palmer E, Weissman IL. Characterization of a rat monoclonal antibody specific for a determinant encoded by the V beta 7 gene segment. Depletion of V beta 7+ T cells in mice with Mls-1a haplotype. J Immunol. 1990; 144(9):3473-3477. (Immunogen). View Reference
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Shinohara K, Ikarashi Y, Maruoka H, et al. Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains. Eur J Immunol. 1999; 29(6):1871-1878. (Biology). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.