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      PE Mouse Anti-Human S100A9 (MRP-14)
      PE Mouse Anti-Human S100A9 (MRP-14)
      Flow cytometric analysis of S100A9 (MRP-14) expression in human peripheral blood monocytes or granulocytes. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 550617; dashed line histograms) or PE Mouse Anti-Human S100A9 (MRP-14) antibody (Cat. No. 565793; solid line histograms). The fluorescence histograms showing expression of S100A9 (MRP-14) or [Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood monocytes (Left Plot) or granulocytes (Right Plot). Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      PE Mouse Anti-Human S100A9 (MRP-14)
      Flow cytometric analysis of S100A9 (MRP-14) expression in human peripheral blood monocytes or granulocytes. Human peripheral blood was treated with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899) to lyse erythrocytes, washed, and then fixed and permeabilized with BD Cytofix/Cytoperm™ Fixation and Permeabilization Solution (Cat. No. 554722). The cells were washed and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with either PE Mouse IgG1, κ Isotype Control (Cat. No. 550617; dashed line histograms) or PE Mouse Anti-Human S100A9 (MRP-14) antibody (Cat. No. 565793; solid line histograms). The fluorescence histograms showing expression of S100A9 (MRP-14) or [Ig Isotype control staining] were derived from gated events with the forward and side light-scatter characteristics of viable human peripheral blood monocytes (Left Plot) or granulocytes (Right Plot). Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
      Product Details
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      BD Pharmingen™
      MRP14; CAGB; CFAG; CGLB; L1AG; LIAG; MAC387; MIF; NIF; P14; 60B8AG
      Human (QC Testing)
      Mouse IgG1, κ
      Human MRP-14 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      5 µl
      AB_2739358
      Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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      Product Notices

      1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      6. An isotype control should be used at the same concentration as the antibody of interest.
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      565793 Rev. 1
      Antibody Details
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      1H9

      The 1H9 monoclonal antibody specifically binds to S100 calcium binding protein A9 (S100A9), which is also known as Migration inhibitory factor-related protein 14 (MRP-14), Calgranulin-B (CAGB), Calprotectin L1H subunit, or Leukocyte L1 complex heavy chain. S100A9 is an ~14 kDa member of the S100 family of EF-hand calcium-binding proteins. S100A9 is expressed in granulocytes, monocytes, and some secretory epithelial cells. A soluble form of S100A9 can also be found in the serum. S100A9 functions as a chemoattractant for neutrophils and monocytes. S100A9 can associate with another S100 family member, S100A8 (also known as, MRP-8) to form a MRP-8/14 heterodimer called Calprotectin or Cystic fibrosis antigen (CFAG). The heterodimer has a wide range of functions, such as playing roles in arachidonic acid metabolism and stimulation of the innate immune system through its interaction with CD284 (Toll-like receptor 4/TLR4).

              

      565793 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      565793 Rev.1
      Citations & References
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      Development References (3)

      1. Hessian PA, Edgeworth J, Hogg N. MRP-8 and MRP-14, two abundant Ca(2+)-binding proteins of neutrophils and monocytes.. J Leukoc Biol. 1993; 53(2):197-204. (Biology). View Reference
      2. Kerkhoff C, Sorg C, Tandon NN, Nacken W. Interaction of S100A8/S100A9-arachidonic acid complexes with the scavenger receptor CD36 may facilitate fatty acid uptake by endothelial cells.. Biochemistry. 2001; 40(1):241-8. (Biology). View Reference
      3. Robinson MJ, Tessier P, Poulsom R, Hogg N. The S100 family heterodimer, MRP-8/14, binds with high affinity to heparin and heparan sulfate glycosaminoglycans on endothelial cells.. J Biol Chem. 2002; 277(5):3658-65. (Immunogen: ELISA, Radioimmunoassay, Western blot). View Reference
      565793 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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