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BD Pharmingen™ PE Hamster Anti-Mouse Podoplanin
Clone 8.1.1 (RUO)
Flow cytometric analysis of Podoplanin expressed on mouse C2C12 cells. Cells from the mouse C2C12 (Myoblast, ATCC CRL-1772) cell line were harvested with trypsin-EDTA dissociation solution. The cells were then washed and stained with either PE Hamster IgG2, κ Isotype Control (Cat. No. 550085; dashed line histogram) or PE Hamster Anti-Mouse Podoplanin antibody (Cat. No. 566390; solid line histogram) at 0.25 μg/ml. The histogram showing Podoplanin expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable C2C12 cells. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of Podoplanin expressed on mouse C2C12 cells. Cells from the mouse C2C12 (Myoblast, ATCC CRL-1772) cell line were harvested with trypsin-EDTA dissociation solution. The cells were then washed and stained with either PE Hamster IgG2, κ Isotype Control (Cat. No. 550085; dashed line histogram) or PE Hamster Anti-Mouse Podoplanin antibody (Cat. No. 566390; solid line histogram) at 0.25 μg/ml. The histogram showing Podoplanin expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable C2C12 cells. Flow cytometric analysis was performed using a BD™ Canto II Flow Cytometer System. Data shown on this Technical Data Sheet are not lot specific.
Product Details
BD Pharmingen™
Pdpn; Aggrus; Glycoprotein 38; Gp38; OTS-8; RANDAM-2; T1A; T1alpha; PA2.26
Mouse (QC Testing)
Hamster IgG2
Mouse Thymic Stromal 186 Cell Line
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739721
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs. A table of the hamster IgG groups, Reactivity of Mouse Anti-Hamster Ig mAbs, may be viewed at http://www.bdbiosciences.com/documents/hamster_chart_11x17.pdf.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
PE Hamster IgG2, κ Isotype Control RUO
Size
0.1 mg
Cat No.
550085
566390 Rev. 1
Antibody Details
8.1.1
The 8.1.1 monoclonal antibody specifically recognizes Podoplanin which is encoded by Pdpn. Podoplanin is a ~43 kDa type I transmembrane glycoprotein that is also known as Glycoprotein 38 (Gp38), OTS-8 (Ots8), Aggrus, RANDAM-2, or T1alpha (T1A). This heavily glycosylated mucin type protein is named for its expression on kidney glomerular epithelial cells known as podocytes. It is also expressed on epithelial and mesothelial cells including intestinal and thymic epithelial cells, alveolar type I cells, fibroblastic reticular cells, lymphatic endothelial cells, macrophages and osteoblasts. Podoplanin plays an essential role in the development of the heart, lymphatic system, and lungs. Podoplanin is involved in actin cytoskeleton organization, and cellular adhesion and migration. It may also play roles in platelet aggregation, promoting inflammatory diseases, tumorigenesis and cancer cell motility and metastasis.
566390 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
496 nm, 566 nm
576 nm
566390 Rev.1
Citations & References
View product citations for antibody "566390" on CiteAb
Development References (5)
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Astarita JL, Acton SE, Turley SJ. Podoplanin: emerging functions in development, the immune system, and cancer.. Front Immunol. 2012; 3:283. (Biology). View Reference
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Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus. J Histochem Cytochem. 1992; 40(5):651-664. (Immunogen: Flow cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot). View Reference
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Farr AG, Berry ML, Kim A, Nelson AJ, Welch MP, Aruffo A. Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues.. J Exp Med. 1992; 176(5):1477-82. (Clone-specific: Electron microscopy, Immunohistochemistry, Western blot). View Reference
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Malhotra D, Fletcher AL, Astarita J, et al. Transcriptional profiling of stroma from inflamed and resting lymph nodes defines immunological hallmarks.. Nat Immunol. 2012; 13(5):499-510. (Clone-specific: Flow cytometry). View Reference
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Vigl B, Aebischer D, Nitschké M, et al. Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner.. Blood. 2011; 118(1):205-15. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
566390 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.