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FITC Rat Anti-Mouse CD26
FITC Rat Anti-Mouse CD26

Two-color analysis of the expression of CD26 on mouse thymocytes. A single-cell suspension of C57BL/6 thymus was simultaneously stained with PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), PE-conjugated 53-5.8 (anti-mouse CD8b.2, Cat. no. 553041, both panels), and FITC-conjugated H194-112 (Right panel) monoclonal antibodies. Flow cytometry was performed on a FACScanª (BDIS, San Jose, CA).

Two-color analysis of the expression of CD26 on mouse thymocytes. A single-cell suspension of C57BL/6 thymus was simultaneously stained with PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), PE-conjugated 53-5.8 (anti-mouse CD8b.2, Cat. no. 553041, both panels), and FITC-conjugated H194-112 (Right panel) monoclonal antibodies. Flow cytometry was performed on a FACScanª (BDIS, San Jose, CA).

Product Details
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BD Pharmingen™
Cd26; Dpp4; dipeptidylpeptidase 4; THAM, DPP IV
Mouse (QC Testing)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2a, κ
Cell-surface molecules solubilized from BALB/c mouse thymocytes
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_397295
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
559652 Rev. 10
Antibody Details
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H194-112

The H194-112 monoclonal antibody specifically binds to CD26, which is also known as, Thymocyte-activating molecule (THAM), or dipeptidyl peptidase IV (DPP IV, Dpp4).  CD26 is a ~220- kDa dimer formed of identical type-II transmembrane core polypeptides which undergo variable post-translational modifications.  It is a multi-functional molecule with both ectopeptidase and signal-transducing activities.  Studies with specific DPP IV inhibitors suggest that the enzymatic activity is involved in the mediation of T-cell activation events.  The expression of CD26 is developmentally regulated in the thymus.  Resting lymphoid cells of the bone marrow and peripheral B and T lymphocytes express low levels of CD26; bone-marrow and peritoneal myeloid cells do not.  CD26 is also found on epithelial cells in the kidney, liver, small intestine, and lung.  Cross-linked H194-112 mAb induces proliferation of immature and mature thymocytes in the presence of either IL-1 plus IL-2 or PMA;  addition of IL-2 or IL-4 to PMA further enhances the activation.

559652 Rev. 10
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
559652 Rev.10
Citations & References
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Development References (7)

  1. Fleischer B. CD26: a surface protease involved in T-cell activation. Immunol Today. 1994; 15(4):180-184. (Biology). View Reference
  2. Gorvel JP, Vivier I, Naquet P, Brekelmans P, Rigal A, Pierres M. Characterization of the neutral aminopeptidase activity associated to the mouse thymocyte-activating molecule. J Immunol. 1990; 144(8):2899-2907. (Biology). View Reference
  3. Marguet D, Bernard AM, Vivier I, Darmoul D, Naquet P, Pierres M. cDNA cloning for mouse thymocyte-activating molecule. A multifunctional ecto-dipeptidyl peptidase IV (CD26) included in a subgroup of serine proteases. J Biol Chem. 1992; 267(4):2200-2208. (Biology). View Reference
  4. Naquet P, MacDonald HR, Brekelmans P. A novel T cell-activating molecule (THAM) highly expressed on CD4-CD8- murine thymocytes. J Immunol. 1988; 141(12):4101-4109. (Immunogen: Activation). View Reference
  5. Naquet P, Vivier I, Gorvel JP. Activation of mouse T lymphocytes by a monoclonal antibody to a developmentally regulated surface aminopeptidase (THAM). Immunol Rev. 1989; 111:177-193. (Clone-specific: Activation). View Reference
  6. Reinhold D, Bank U, Buhling F. Inhibitors of dipeptidyl peptidase IV (DP IV, CD26) induces secretion of transforming growth factor-beta 1 (TGF-beta 1) in stimulated mouse splenocytes and thymocytes. Immunol Lett. 1997; 58(1):29-35. (Biology: Activation). View Reference
  7. Vivier I, Marguet D, Naquet P . Evidence that thymocyte-activating molecule is mouse CD26 (dipeptidyl peptidase IV). J Immunol. 1988; 147(2):447-454. (Biology). View Reference
View All (7) View Less
559652 Rev. 10

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.