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BV421 Rat Anti-Mouse CD13
BV421 Rat Anti-Mouse CD13

Two-color flow cytometric analysis of CD13 expression by mouse splenocytes (left and center panels). Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD11b antibody (Cat. No. 553311/557397/561689) and either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; Left Panel) or BD Horizon BV421 Rat Anti-Mouse CD13 antibody (Cat. No. 564354; Center Panel).  Two-color contour plots showing the correlated expression of CD13 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Immunohistofluorescent analysis of CD13 expression by cells within C57BL/6 mouse spleen (right panel). A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Horizon™ BV421 Rat Anti-Mouse CD13 antibody (Cat. No. 564354, pseudo-colored green) and Alexa Fluor® 647 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 557869, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

Two-color flow cytometric analysis of CD13 expression by mouse splenocytes (left and center panels). Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD11b antibody (Cat. No. 553311/557397/561689) and either BD Horizon™ BV421 Rat IgG1, κ Isotype Control (Cat. No. 562868; Left Panel) or BD Horizon BV421 Rat Anti-Mouse CD13 antibody (Cat. No. 564354; Center Panel).  Two-color contour plots showing the correlated expression of CD13 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Immunohistofluorescent analysis of CD13 expression by cells within C57BL/6 mouse spleen (right panel). A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with BD Horizon™ BV421 Rat Anti-Mouse CD13 antibody (Cat. No. 564354, pseudo-colored green) and Alexa Fluor® 647 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 557869, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

Product Details
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BD Horizon™
Anpep; Alanyl aminopeptidase; Aminopeptidase N; AP-N; mAPN; Lap-1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG1
Crude Aminopeptidase N Preparation from BALB/c Mouse Intestinal Epithelium
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_2738764
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  8. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  10. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564354 Rev. 2
Antibody Details
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R3-242

The R3-242 monoclonal antibody specifically binds to aminopeptidase N (APN), which has been reported to be the mouse CD13 homologue. APN is expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, R3-242 mAb is reported to detect APN on monocytes, macrophages, mast cells, dendritic cells, and B lymphocytes, but not on T cells, and thymocytes. However, the reported reactivity with B cells may be non-specific, via binding to the Fcγ receptors (CD16/CD32). This observation is in agreement with reports using different antibodies to mouse APN. APN may play a role in antigen presentation in the immune system. R3-242 mAb does not inhibit the enzymatic activity of APN.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

564354 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
564354 Rev.2
Citations & References
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Development References (4)

  1. Chen H, Kinzer CA, Paul WE. p161, a murine membrane protein expressed on mast cells and some macrophages, is mouse CD13/aminopeptidase N. J Immunol. 1996; 157(6):2593-2600. (Clone-specific: Flow cytometry). View Reference
  2. Hansen AS, Noren O, Sjostrom H, Werdelin O. A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules. Eur J Immunol. 1993; 23(9):2358-2364. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
  3. Leenen PJ, Melis M, Kraal G, Hoogeveen AT, Van Ewijk W. The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity. Eur J Immunol. 1992; 22(6):1567-1572. (Biology). View Reference
  4. Pulendran B, Lingappa J, Kennedy MK, et al. Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice. J Immunol. 1997; 159(5):2222-2231. (Biology). View Reference
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564354 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.