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Flow cytometric analysis of CXCL9 (MIG) expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 (Macrophage; ATCC® TIB-71™) cells were cultured (2 hours) without [Unstimulated; dashed line histogram] or with [Stimulated; solid line histogram] Recombinant Mouse IFN-γ protein (Cat. No. 554587; 10 ng/ml) followed by LPS (1 μg/ml). BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) was added for overnight culture. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Hamster Anti-Mouse CXCL9 (MIG) antibody (Cat. No. 570803/570879) at 0.5 μg/test. The histograms showing CXCL9 (MIG) expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.


BD Horizon™ BV421 Hamster Anti-Mouse CXCL9 (MIG)

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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
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The MIG 2F5.5 monoclonal antibody specifically recognizes MIG (Monokine induced by gamma interferon) which is also known as C-X-C motif chemokine 9 (CXCL9) or Small-inducible cytokine B9 (Scyb9). Mouse CXCL9 (MIG) is encoded by Cxcl9 and belongs to the C-X-C family of chemokines. CXCL9 (MIG) production is induced in a variety of cells including macrophages, hepatocytes, and endothelial cells in response to gamma interferon (IFN-γ). The seven-transmembrane G-protein coupled receptor termed CXCR3, also known as CD183, serves as the cell surface signaling receptor for CXCL9 (MIG). This chemotactic factor attracts activated and memory CD4+ and CD8+ T cells including Type-1 (Th1-like) CD4+ T cells as well as natural killer (NK) cells. CXCL9 (MIG) plays important roles in promoting protective immunity through the attraction of CXCR3+ T cells and NK cells but can also function in inflammation and autoimmune diseases.

Development References (4)
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Asai A, Tsuda Y, Kobayashi M, Hanafusa T, Herndon DN, Suzuki F. Pathogenic role of macrophages in intradermal infection of methicillin-resistant Staphylococcus aureus in thermally injured mice.. Infect Immun. 2010; 78(10):4311-9. (Clone-specific: Flow cytometry). View Reference
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Krug A, Uppaluri R, Facchetti F, et al. IFN-producing cells respond to CXCR3 ligands in the presence of CXCL12 and secrete inflammatory chemokines upon activation. J Immunol. 2002; 169(11):6079-6083. (Immunogen: ELISA). View Reference
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Marcus AJ, Ullman HL, Safier LB. Lipid composition of subcellular particles of human blood platelets.. J Lipid Res. 1969; 10(1):108-14. (Biology). View Reference
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Sung JH, Zhang H, Moseman EA, et al. Chemokine guidance of central memory T cells is critical for antiviral recall responses in lymph nodes.. Cell. 2012; 150(6):1249-63. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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