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BV421 Hamster Anti-Mouse CXCL9 (MIG)

BD Horizon™ BV421 Hamster Anti-Mouse CXCL9 (MIG)

Clone MIG-2F5.5 (also known as 2F.5.5.5)

(RUO)
BV421 Hamster Anti-Mouse CXCL9 (MIG)
Flow cytometric analysis of CXCL9 (MIG) expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 (Macrophage; ATCC® TIB-71™) cells were cultured (2 hours) without [Unstimulated; dashed line histogram] or with [Stimulated; solid line histogram] Recombinant Mouse IFN-γ protein (Cat. No. 554587; 10 ng/ml) followed by LPS (1 μg/ml). BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) was added for overnight culture. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Hamster Anti-Mouse CXCL9 (MIG) antibody (Cat. No. 570803/570879) at 0.5 μg/test. The histograms showing CXCL9 (MIG) expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Flow cytometric analysis of CXCL9 (MIG) expression in Unstimulated and Stimulated Mouse RAW264.7 cells. RAW 264.7 (Macrophage; ATCC® TIB-71™) cells were cultured (2 hours) without [Unstimulated; dashed line histogram] or with [Stimulated; solid line histogram] Recombinant Mouse IFN-γ protein (Cat. No. 554587; 10 ng/ml) followed by LPS (1 μg/ml). BD GolgiPlug™ Protein Transport Inhibitor (Cat. No. 555029) was added for overnight culture. Cells were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with BD Horizon™ BV421 Hamster Anti-Mouse CXCL9 (MIG) antibody (Cat. No. 570803/570879) at 0.5 μg/test. The histograms showing CXCL9 (MIG) expression in Unstimulated versus Stimulated cells were overlaid for the sake of direct comparison as shown. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
Cxcl9; Mig; CMK; M119; crg-10; Scyb9
Mouse (QC Testing)
Armenian Hamster IgG, κ
Mouse CXCL9 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. An isotype control should be used at the same concentration as the antibody of interest.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. For U.S. patents that may apply, see bd.com/patents.
570879 Rev. 1
Antibody Details
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MIG-2F5.5

The MIG 2F5.5 monoclonal antibody specifically recognizes MIG (Monokine induced by gamma interferon) which is also known as C-X-C motif chemokine 9 (CXCL9) or Small-inducible cytokine B9 (Scyb9). Mouse CXCL9 (MIG) is encoded by Cxcl9 and belongs to the C-X-C family of chemokines. CXCL9 (MIG) production is induced in a variety of cells including macrophages, hepatocytes, and endothelial cells in response to gamma interferon (IFN-γ). The seven-transmembrane G-protein coupled receptor termed CXCR3, also known as CD183, serves as the cell surface signaling receptor for CXCL9 (MIG). This chemotactic factor attracts activated and memory CD4+ and CD8+ T cells including Type-1 (Th1-like) CD4+ T cells as well as natural killer (NK) cells. CXCL9 (MIG) plays important roles in promoting protective immunity through the attraction of CXCR3+ T cells and NK cells but can also function in inflammation and autoimmune diseases.

570879 Rev. 1
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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BV421
Violet 405 nm
407 nm
423 nm
570879 Rev.1
Citations & References
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View product citations for antibody "570879" on CiteAb

Development References (4)

  1. Asai A, Tsuda Y, Kobayashi M, Hanafusa T, Herndon DN, Suzuki F. Pathogenic role of macrophages in intradermal infection of methicillin-resistant Staphylococcus aureus in thermally injured mice.. Infect Immun. 2010; 78(10):4311-9. (Clone-specific: Flow cytometry). View Reference
  2. Krug A, Uppaluri R, Facchetti F, et al. IFN-producing cells respond to CXCR3 ligands in the presence of CXCL12 and secrete inflammatory chemokines upon activation. J Immunol. 2002; 169(11):6079-6083. (Immunogen: ELISA). View Reference
  3. Marcus AJ, Ullman HL, Safier LB. Lipid composition of subcellular particles of human blood platelets.. J Lipid Res. 1969; 10(1):108-14. (Biology). View Reference
  4. Sung JH, Zhang H, Moseman EA, et al. Chemokine guidance of central memory T cells is critical for antiviral recall responses in lymph nodes.. Cell. 2012; 150(6):1249-63. (Clone-specific: Flow cytometry, Immunofluorescence). View Reference
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570879 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.