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Alexa Fluor® 647 Rat Anti-Mouse CD13
Alexa Fluor® 647 Rat Anti-Mouse CD13

Two-color flow cytometric analysis of CD13 expression by mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD11b antibody (Cat. No. 553311/557397/561689) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD13 antibody (Cat. No. 564352; Right Panel).  Two-color contour plots showing the correlated expression of CD13 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Alexa Fluor® 647 Rat Anti-Mouse CD13

Immunohistofluorescent analysis of CD13 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 647 Rat Anti-Mouse CD13 antibody (Cat. No. 564352, pseudo-colored green) and BD Horizon™ BV421 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 564008, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

Two-color flow cytometric analysis of CD13 expression by mouse splenocytes. Mouse splenic leucocytes were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) (Cat. No. 553141/553142). The cells were then stained with PE Rat Anti-Mouse CD11b antibody (Cat. No. 553311/557397/561689) and either Alexa Fluor® 647 Rat IgG1, κ Isotype Control (Cat. No. 557731; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse CD13 antibody (Cat. No. 564352; Right Panel).  Two-color contour plots showing the correlated expression of CD13 (or Ig Isotype control staining) versus CD11b were derived from gated events with the forward and side light-scatter characteristics of viable leucocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

Immunohistofluorescent analysis of CD13 expression by cells within C57BL/6 mouse spleen. A mouse spleen cryosection (5 µm) was fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), blocked with 5% goat serum and 1% BSA diluted in 1x PBS, and stained with Alexa Fluor® 647 Rat Anti-Mouse CD13 antibody (Cat. No. 564352, pseudo-colored green) and BD Horizon™ BV421 Rat Anti-Mouse CD3 Molecular Complex antibody (Cat. No. 564008, pseudo-colored red). Images were captured on a standard epifluorescence microscope. Original magnification, 20x.

Product Details
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BD Pharmingen™
Anpep; Alanyl aminopeptidase; Aminopeptidase N; AP-N; mAPN; Lap-1
Mouse (QC Testing)
Rat LEW, also known as Lewis IgG1
Crude Aminopeptidase N Preparation from BALB/c Mouse Intestinal Epithelium
Flow cytometry (Routinely Tested), Immunofluorescence (Tested During Development)
0.2 mg/ml
AB_2738763
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  5. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
564352 Rev. 2
Antibody Details
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R3-242

The R3-242 monoclonal antibody specifically binds to aminopeptidase N (APN), which has been reported to be the mouse CD13 homologue. APN is expressed in a variety of mammalian tissues, including intestinal and renal epithelial brush border, endothelium, liver, lung, and brain. In lymphoid tissues, R3-242 mAb is reported to detect APN on monocytes, macrophages, mast cells, dendritic cells, and B lymphocytes, but not on T cells, and thymocytes. However, the reported reactivity with B cells may be non-specific, via binding to the Fcγ receptors (CD16/CD32). This observation is in agreement with reports using different antibodies to mouse APN. APN may play a role in antigen presentation in the immune system. R3-242 mAb does not inhibit the enzymatic activity of APN.

564352 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
564352 Rev.2
Citations & References
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Development References (4)

  1. Chen H, Kinzer CA, Paul WE. p161, a murine membrane protein expressed on mast cells and some macrophages, is mouse CD13/aminopeptidase N. J Immunol. 1996; 157(6):2593-2600. (Clone-specific: Flow cytometry). View Reference
  2. Hansen AS, Noren O, Sjostrom H, Werdelin O. A mouse aminopeptidase N is a marker for antigen-presenting cells and appears to be co-expressed with major histocompatibility complex class II molecules. Eur J Immunol. 1993; 23(9):2358-2364. (Immunogen: Blocking, ELISA, Flow cytometry, Functional assay, Immunoprecipitation, Radioimmunoassay). View Reference
  3. Leenen PJ, Melis M, Kraal G, Hoogeveen AT, Van Ewijk W. The monoclonal antibody ER-BMDM1 recognizes a macrophage and dendritic cell differentiation antigen with aminopeptidase activity. Eur J Immunol. 1992; 22(6):1567-1572. (Biology). View Reference
  4. Pulendran B, Lingappa J, Kennedy MK, et al. Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice. J Immunol. 1997; 159(5):2222-2231. (Biology). View Reference
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564352 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.