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      Purified Mouse Anti-Human Syk

      BD Pharmingen™ Purified Mouse Anti-Human Syk

      (RUO)
      Purified Mouse Anti-Human Syk
      Western blot analysis of Syk. Lysate from EB1 cells was probed with anti-Syk (clone 4D10) at concentrations of 0.125 (lane 1), 0.063 (lane 2), and 0.031 µg/ml (lane 3). Syk is identified as a band of ~72 kDa.
      Purified Mouse Anti-Human Syk Purified Mouse Anti-Human Syk
      Western blot analysis of Syk. Lysate from EB1 cells was probed with anti-Syk (clone 4D10) at concentrations of 0.125 (lane 1), 0.063 (lane 2), and 0.031 µg/ml (lane 3). Syk is identified as a band of ~72 kDa.
      Product Details
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      BD Pharmingen™
      Human (QC Testing)
      Western blot (Routinely Tested), Flow cytometry (Tested During Development)
      RUO
      AB_394267


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.
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      Recommended Assay Procedures

      Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).

      Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis. Cat. No. 552476 is recommended for use in flow cytometry.

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
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      551817 Rev. 1
      Components
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      Description Quantity/Size Part Number EntrezGene ID
      EB1 Control Lysate 50 µg (1 ea) 51-16626N N/A
      Purified Mouse Anti-Human Syk 50 µg (1 ea) 51-8116GR N/A
      551817 Rev. 1
      Citations & References
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      Development References (4)

      1. Chu DH, van Oers NS, Malissen M, Harris J, Elder M, Weiss A. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol. 1999; 163(5):2610-2620. (Immunogen: Flow cytometry, Western blot). View Reference
      2. Costello PS, Turner M, Walters AE, et al. Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells. Oncogene. 1996; 13(12):2595-2605. (Biology). View Reference
      3. Latour S, Veillette A. Proximal protein tyrosine kinases in immunoreceptor signaling. Curr Opin Immunol. 2001; 13:299-306. (Biology).
      4. Turner M, Schweighoffer E, Colucci F, Di Santo JP, Tybulewicz VL. Tyrosine kinase SYK; essential functions for immunoreceptor signaling. Immunol Today. 2000; 21:148-154. (Biology). View Reference
      551817 Rev. 1

      Please refer to Support Documents for Quality Certificates

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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