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      PE Mouse Anti-Human IL-1β
      Product Details
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      BD FastImmune™
      IL-1 beta
      Human
      Mouse BALB/c IgG1, κ
      Human IL-1β
      Flow cytometry
      20 μL
      Phosphate buffered saline with gelatin and 0.1% sodium azide.
      RUO (GMP)


      Preparation And Storage

      The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

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      340516 Rev. 2
      Antibody Details
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      AS10

      The AS10 antibody reacts with human interleukin-1β (IL-1β) which is also known as endogenous pyrogen (EP), leukocyte endogenous mediator (LEM), mononuclear cell factor (MCF) and lymphocyte-activating factor (LAF). IL-1β is a proinflammatory cytokine that is synthesized as a precursor of 31 kDa and is converted intracellularly to the mature 17.5 kDa form, after cleavage by the IL-1β-converting enzyme (ICE). In healthy individuals, IL-1β is secreted non-constitutively by blood monocytes, tissue macrophages and dendritic cells. IL-1β is also constitutively expressed in the human hypothalamus. Many malignant tumors express IL-1β as part of their neoplastic nature.  The AS10 antibody has been reported not to recognize human IL-1α nor cross react with mouse IL-1β.

      340516 Rev. 2
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      340516 Rev.2
      Citations & References
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      View product citations for antibody "340516" on CiteAb

      Development References (11)

      1. Conlon PJ, Grabstein KH, Alpert A, et al. Localization of human mononuclear cell interleukin 1. J Immunol. 1987; 139:98-102. (Biology).
      2. Dinarello CA. Biology of interleukin 1. FASEB J. 1988; 2:108-115. (Biology).
      3. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
      4. Giri JG, Lomedico PT, Mizel SB. Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1. J Immunol. 1985; 134(1):343-349. (Biology). View Reference
      5. Mantovani A, Dejana E. Cytokines as communication signals between leukocytes and endothelial cells.. Immunol Today. 1989; 10(11):370-5. (Biology). View Reference
      6. Matsushima K, Yodoi J, Tagaya Y, Oppenheim JJ. Downregulation of interleukin-1 receptor expression by IL-1 and fate of internalized125 I-labeled IL-1 β in a human large granular lymphocyte cell line. J Immunol. 1986; 137:3183-3188. (Biology).
      7. Mizel SB. The interleukins. FASEB J. 1989; 32379-2388. (Biology).
      8. Saklatvala J. Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Science. 1986; 322:547-549. (Biology).
      9. Singer II, Scott S, Chin J, et al. The Interleukin-1 β –converting enzyme (ICE) is localized on the external cell surface membranes and in the cytoplasmic ground substance of human monocytes by immuno-electron microscopy. J Exp Med. 1995; 182:1447-1459. (Biology).
      10. Slack J, McMahan CJ, Waugh S. et al. J Bio Chem. 1989; 10:370-375. (Biology).
      11. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
      View All (11) View Less
      340516 Rev. 2

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.