Description
The CycleTEST™ PLUS DNA Reagent Kit provides a set of re agents for isolating and staining cell nuclei from surplus fresh or frozen solid tissue specimens or cell suspensions. Flow cytometric analysis of differentially stained normal and tumor cells is used for research in the identification of abnormal DNA stemlines and to estimate the DNA index (DI) and cell-cycle phase distributions of these stemlines. Contents: Trypsin solution, trypsin inhibitor/ribonuclease A solution, propidium iodide solution.
Preparation And Storage
1. For research use only. Not for use in diagnostic or therapeutic procedures.
2. When stored at -18°C, the reagents are stable until the expiration date shown on the C ycleTEST PLUS kit label. Do not use after the expiration date. The thawed reagents are stable for 1 m onth when stored at 2° to 8°C.
3. The reagents should not be refrozen after thawing. Do not ex pose reagents to direct light during storage or during incubation with cells.
4. Reagents should not be heated to 37°C although they may be briefly exposed to a 37°C water bath in the process of thawing.
5. Solution C (PI) should be protected from light and kept ice cold (2° to 8°C).
6. Incubation times, ce ntrifugation times, or temperatures other than those specified may be a source of error. 7. For optimal results, analyze stained specimens within 3 hours of staining. 8. Alteration in the appearance of the reagents, such as precipitation or discoloration, indicates instability or de terioration. In suc h cases the reagent(s) should not be used.
| Description | Quantity/Size | Part Number | EntrezGene ID |
|---|---|---|---|
| Trypsin Solution | N/A | 91-0204 | N/A |
| Ttrypsin Inhibitor/Ribonuclease A Solution | N/A | 91-0205 | N/A |
| Propidium Iodide Solution | N/A | 91-0206 | N/A |
| Citrate Buffer | N/A | 91-0203 | N/A |
Development References (19)
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Vindelov LL, Ch ristensen IJ, Nissen NI. A det ergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. 1983; 3:323-327. (Biology).
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Vindelov LL, Christensen IJ, Jensen G, Nissen NI. Vindelov LL, Christensen IJ, Jensen G, Nissen NI. Cytometry. 1983; 3:332-339. (Biology).
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Wolley RC, Schreiber K, Koss LG, Karas M, Sherman A. DNA distribution in human colon carcinomas and its relationship to clinical behavior. JNCI. 1982; 69:15-22. (Biology).
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Although not required, these products are manufactured in accordance with Good Manufacturing Practices.
