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CD8 Intracellular Cytokine Detection Kit Anti-Hu-IFN-γ/CD69/CD8/CD3

CD8 Intracellular Cytokine Detection Kit Anti-Hu-IFN-γ/CD69/CD8/CD3

(RUO (GMP))
Product Details
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BD FastImmune™
Human
Intracellular staining (flow cytometry)
RUO (GMP)


Description

The BD FastImmune™ CD8 Intracellular Cytokine Detection Kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD8+ T lymphocytes in whole blood. Applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.

Contents: Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Solution, EDTA Solution, BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x).


The BD FastImmune™ CD8 Intracellular Cytokine Detection Kit is designed for the detection of intracellular cytokines and the activation marker CD69 in antigen-activated CD8+ T lymphocytes in whole blood. Applications include studies of T-cell responses to antigens, such as herpes viruses, HIV, and tumor antigens.

Contents: Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC), Isotype Ctrl Cocktail, Co-Stimulatory Antibodies (CD28/CD49d), Brefeldin A (BFA) Solution, EDTA Solution, BD FACS™ Lysing Solution (10x), BD FACS Permeabilizing Solution 2 (10x).

Preparation And Storage

Upon receipt, thaw BFA, dispense into 10 µL aliquots, and store at –20°C.

Store each kit at 2° to 8°C.

BD FACS Lysing Solution (10X) and BD FACS Permeabilizing Solution 2 (10X) are each stable for the period shown on the appropriate bottle label when stored as directed. Do not use either reagent if discoloration occurs or a precipitate forms. Dilute 1:10 in deionized (DI) water. Use at room temperature. When stored at 2° to 8°C, each antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

346049 Rev. 1
Components
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Description Quantity/Size Part Number
Antibody Cocktail (IFN-γ FITC, CD69 PE, CD8 PerCP-Cy™5.5, CD3 APC) 50 Tests (1 ea) 346048
Isotype Ctrl Cocktail 50 Tests (1 ea) 346047
Brefeldin A (BFA) Solution N/A 347688
Fastimmune Intracellular Cytokine Detection Kit 25 Tests (1 ea) 91-0457
346049 Rev. 1
Citations & References
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Development References (20)

  1. Asanuma H, Sharp M, Maecker HT, Maino VC, Arvin AM. Frequencies of memory T cells specific for varicella-zoster virus, herpes simplex virus, and cytomegalovirus determined by intracellular detection of cytokine expression. J Infec Dis. 2000; 181:859-866. (Biology).
  2. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. NCCLS document H42-A. 1998. (Biology).
  3. Ghanekar SA, Nomura LE, Suni MA, Picker LJ, Maecker HT, Maino VC. γ interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65. Clin Diagn Lab Immunol. 2001; 8:628-631. (Biology).
  4. He XS, Rehermann B, Lopez-Labrador FX, et al. Quantitative analysis of hepatitis C virus-specific CD8+ T cells in peripheral blood and liver using peptide-MHC tetramers. Proc Natl Acad Sci USA. 1999; 96:5692-5697. (Biology).
  5. Karanikas V, Lodding J, Maino VC, McKenzie IFC. Flow cytometric measurement of intracellular cytokines detects immune responses in MUCI immunotherapy. Clin Cancer Res. 2000; 6:829-837. (Biology).
  6. Kaul R, Dong T, Plummer FA, et al. CD8+ lymphocytes respond to different HIV epitopes in seronegative and infected subjects. J Clin Invest. 2001; 107:1303-1310. (Biology).
  7. Kaul R, Plummer FA, Kimani J, et al. HIV-1-specific mucosol CD8+ lymphocyte responses in the cervix of HIV-1-resistant prostitues in Nairobi. J Immunol. 2000; 164:341-349. (Biology).
  8. Kaul R, Rowland-Jones SL, Kimani J, et al. Late seroconversion in HIV-resistant Nairobi prostitutes despite pre-existing HIV-specific CD8+ responses. J Clin Invest. 2001; 107:341-349. (Biology).
  9. Kern F, Faulhaber N, Fruömmel C, et al. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentadecapeptides. Eur J Immunol. 2000; 30:1676-1682. (Biology).
  10. Kern F, Surel IP, Brock C, et al. T-cell epitope mapping by flow cytometry. Nat Med. 1998; 4:975-978. (Biology).
  11. Kuzushima K, Hoshino Y, Fufii K, et al. Rapid determination of Epstein-Barr–specific CD8+ T-cell frequencies by flow cytometry. Blood. 1999; 94:3094-3100. (Biology).
  12. Maecker HT, Dunn HS, Suni MA, et al. Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods. 2001; 255:27-40. (Biology).
  13. Maecker HT, Ghanekar SZ, Suni MA, HE X-S, Picker LJ, Maino VC. Factors affecting the efficiency of CD8+ T cell cross-priming with exogenous antigens. J Immunol. 2001; 166:7268-7275. (Biology).
  14. Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression.. Cytometry. 1998; 34(5):207-15. (Biology). View Reference
  15. Maino VC. Rapid assessment of antigen induced cytokine expression in memory T cells by flow cytometry.. Vet Immunol Immunopathol. 1998; 63(1-2):199-207. (Biology). View Reference
  16. Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. 2000; 40:60-68. (Biology).
  17. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline. NCCLS document M29-T2. (Biology).
  18. Schmitz JE, Kuroda MJ, Santra S, et al. Control of viremia in simian immunodeficiency virus infection by CD8+ lymphocytes. Science. 1999; 238:857-860. (Biology).
  19. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
  20. Waldrop SL, Davis KA, Maino VC, Picker LJ. Normal human CD4+ memory T cells display broad heterogeneity in their activation threshold for cytokine synthesis.. J Immunol. 1998; 161(10):5284-95. (Biology). View Reference
View All (20) View Less
346049 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

 

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