Flow cytometric analysis of Integrin β7 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841; dashed line histogram) or Purified Rat Anti-Integrin β7 antibody (Cat. No. 555943; solid line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Secondary staining was carried out with FITC Goat Anti-Rat Ig (Cat. No. 554016). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Flow cytometric analysis of Integrin β7 expression on human peripheral blood lymphocytes. Whole blood was stained with either Purified Rat IgG2a, κ Isotype Control (Cat. No. 555841; dashed line histogram) or Purified Rat Anti-Integrin β7 antibody (Cat. No. 555943; solid line histogram). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). Secondary staining was carried out with FITC Goat Anti-Rat Ig (Cat. No. 554016). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
Product Details
BD Pharmingen™
Itgb7; Integrin β7; Integrin beta 7; ITB7; Ly-69; Ly69
Human (QC Testing), Mouse (Tested in Development)
Rat F344, also known as Fischer, CDF IgG2a, κ
Mouse T lymphoma line TK1
Flow cytometry (Routinely Tested)
0.5 mg/ml
VI A024, VI 6T-101
AB_396240
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Purified Rat IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
555841
FITC Goat Anti-Rat Ig RUO
Size
0.5 mg
Cat No.
554016
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555943 Rev. 6
Antibody Details
FIB504
The FIB504 monoclonal antibody specifically recognizes mouse integrin β7 subunit (130 kDa) but also crossreacts with human integrin β7. Integrin β7 associates with α4 (CD49d) expressed on subsets of lymphocytes and thymocytes. It also associates with αIEL (CD103) expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Integrin β7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leucocytes to extravascular spaces during the inflammatory response.
555943 Rev. 6
Format Details
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
555943 Rev.6
Citations & References
Development References (7)
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Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Biology). View Reference
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Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Biology). View Reference
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Butcher EC, Picker LJ. Lymphocyte homing and homeostasis. Science. 1996; 272(5258):60-66. (Biology). View Reference
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Erle DJ, Briskin MJ, Butcher EC, Garcia-Pardo A, Lazarovits AI, Tidswell M. Expression and function of the MAdCAM-1 receptor, integrin alpha 4 beta 7, on human leukocytes. J Immunol. 1994; 153(2):517-528. (Biology). View Reference
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Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
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Rott LS, Briskin MJ, Andrew DP, Berg EL, Butcher EC. A fundamental subdivision of circulating lymphocytes defined by adhesion to mucosal addressin cell adhesion molecule-1. Comparison with vascular cell adhesion molecule-1 and correlation with beta 7 integrins and memory differentiation. J Immunol. 1996; 156(10):3727-3736. (Biology). View Reference
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Teague TK, Lazarovits AI, McIntyre BW. Integrin alpha 4 beta 7 co-stimulation of human peripheral blood T cell proliferation. Cell Adhes Commun. 1994; 2(6):539-547. (Biology). View Reference
555943 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
