Preparation And Storage
Recommended Assay Procedures
This antibody has been tested by cell-surface immunofluorescent staining ( ≤8µg/million cells) with flow cytometric analysis to assure specificity and reactivity. Since the pTα is expressed at low density on the surface of immature thymocytes, we recommend the use of Mouse Fc Block™ (anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142) and amplification of the staining intensity through the use of a biotinylated second-step antibody, such as anti-mouse IgG1 mAb A85-1 (Cat. No. 553441), followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. No. 554061).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
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At very early stages in the intrathymic differentiation of T lymphocytes which will bear αβ T Cell Receptors (TCR), the TCR β chain forms a heterodimer with the pre-TCR α chain (pTα) to form the pre-TCR. This pre-TCR associates with the signal-transducing CD3 complex and controls the survival and proliferation of CD4- CD8- (DN) thymocytes, plays a role in allelic exclusion of the TCR β chain, directly or indirectly regulates TCR δ chain expression, and is eventually replaced by the TCR α chain as thymocytes mature. The 2F5 monoclonal antibody specifically recognizes the PTα on the surface of CD44- CD25-/lo DN thymocytes.
Development References (7)
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Aifantis I, Azogui O, Feinberg J, Saint-Ruf C, Buer J, von Boehmer H. On the role of the pre-T cell receptor in alphabeta versus gammadelta T lineage commitment. Immunity. 1998; 9(5):649-688. (Immunogen: Immunoprecipitation). View Reference
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Aifantis I, Feinberg J, Fehling HJ, Di Santo JP, von Boehmer H. Early T cell receptor beta gene expression is regulated by the pre-T cell receptor-CD3 complex. J Exp Med. 1999; 190(1):141-144. (Biology). View Reference
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Aifantis I, Pivniouk VI, Gartner F, et al. Allelic exclusion of the T cell receptor beta locus requires the SH2 domain-containing leukocyte protein (SLP)-76 adaptor protein. J Exp Med. 1999; 190(8):1093-1102. (Biology). View Reference
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Mancini S, Candeias SM, Fehling HJ, von Boehmer H, Jouvin-Marche E, Marche PN. TCR alpha-chain repertoire in pTalpha-deficient mice is diverse and developmentally regulated: implications for pre-TCR functions and TCRA gene rearrangement. J Immunol. 1999; 163(11):6053-6059. (Biology). View Reference
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Wiest DL, Berger MA, Carleton M. Control of early thymocyte development by the pre-T cell receptor complex: A receptor without a ligand. Semin Immunol. 1999; 11(4):251-262. (Biology). View Reference
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von Boehmer H, Aifantis I, Feinberg J, et al. Pleiotropic changes controlled by the pre-T-cell receptor. Curr Opin Immunol. 1999; 11(2):135-142. (Biology). View Reference
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von Boehmer H, Fehling HJ. Structure and function of the pre-T cell receptor. Annu Rev Immunol. 1997; 15:433-452. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.